ArticleChembiochem : a European journal of chemical biology2013
Catch-and-release probes applied to semi-intact cells reveal ubiquitin-specific protease expression in Chlamydia trachomatis infection.
Article in Chembiochem : a European journal of chemical biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Understanding protein ISGylation, a multifaceted posttranslational modification.Cell chemical biology · 2026Review
- Synthetic Strategies for Activity-Based Probes to Decode Ubiquitin-Like Modifiers.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Review
- Cell-Based Covalent-Capture Deubiquitinase Assay for Inhibitor Discovery.ACS pharmacology & translational science · 2024Article
- The acetylase activity of Cdu1 regulates bacterial exit from infected cells by protectingeLife · 2024Article
- Ubiquitin-targeted bacterial effectors: rule breakers of the ubiquitin system.The EMBO journal · 2023Review
- On the Study of Deubiquitinases: Using the Right Tools for the Job.Biomolecules · 2022Review
- Investigation of hub gene associated with the infection of Staphylococcus aureus via weighted gene co-expression network analysis.BMC microbiology · 2021Article
- Assay Systems for Profiling Deubiquitinating Activity.International journal of molecular sciences · 2020Review
- Modification of the host ubiquitome by bacterial enzymes.Microbiological research · 2020Review
- The Two Deubiquitinating Enzymes fromBiochemistry · 2020Article
- The multiple functions of the numerousMicrobial cell (Graz, Austria) · 2019Review
- Recent Developments in Cell Permeable Deubiquitinating Enzyme Activity-Based Probes.Frontiers in chemistry · 2019Review
- Cell-Permeable Activity-Based Ubiquitin Probes Enable Intracellular Profiling of Human Deubiquitinases.Journal of the American Chemical Society · 2018Article
- Orchestration of the mammalian host cell glucose transporter proteins-1 and 3 by Chlamydia contributes to intracellular growth and infectivity.Pathogens and disease · 2017Article
- Activity-based probes for the ubiquitin conjugation-deconjugation machinery: new chemistries, new tools, and new insights.The FEBS journal · 2017Review
- Subunit-Specific Labeling of Ubiquitin Chains by Using Sortase: Insights into the Selectivity of Deubiquitinases.Chembiochem : a European journal of chemical biology · 2016Article
- Chlamydia cell biology and pathogenesis.Nature reviews. Microbiology · 2016Review
- Substrate specificity of the ubiquitin and Ubl proteases.Cell research · 2016Review
- Usp12 stabilizes the T-cell receptor complex at the cell surface during signaling.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Structural basis of substrate recognition by a bacterial deubiquitinase important for dynamics of phagosome ubiquitination.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Protein ubiquitylation controls many cellular pathways, and timely removal of ubiquitin by deubiquitylating enzymes (DUBs) is essential to govern these different functions. To map endogenous expression of individual DUBs as well as that of any interacting proteins, we developed a catch-and-release ubiquitin probe. Ubiquitin was equipped with an activity-based warhead and a cleavable linker attached to a biotin affinity-handle through tandem site-specific modification, in which we combined intein chemistry with sortase-mediated ligation. The resulting probe is cell-impermeable and was therefore delivered to the cytosol of perfringolysin O (PFO)-permeabilized cells. This allowed us to retrieve and identify 34 DUBs and their interacting partners. We also noted the expression, in host cells infected with Chlamydia trachomatis, of two additional DUBs. Furthermore, we retrieved and identified chlamydial DUB1 (ChlaDUB1) and DUB2 (ChlaDUB2), demonstrating by experiment that ChlaDUB2, the presence and activity of which had not been detected in infected cells, is in fact expressed during the course of infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.