ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2013
A trigger channel threshold artifact in nanoparticle analysis.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Characterization of Platelet and Red Blood Cell Extracellular Vesicles by Spectral Flow Cytometry.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential.Brain, behavior, and immunity · 2021Article
- Conventional, High-Resolution and Imaging Flow Cytometry: Benchmarking Performance in Characterisation of Extracellular Vesicles.Biomedicines · 2021Article
- Diameters and Fluorescence Calibration for Extracellular Vesicle Analyses by Flow Cytometry.International journal of molecular sciences · 2020Article
- From laboratory to clinic: Translation of extracellular vesicle based cancer biomarkers.Methods (San Diego, Calif.) · 2020Review
- MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments.Journal of extracellular vesicles · 2020Article
- Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).European journal of immunology · 2019Article
- High-fidelity detection and sorting of nanoscale vesicles in viral disease and cancer.Journal of extracellular vesicles · 2019Article
- Extracellular vesicles and coagulation in blood from healthy humans revisited.Journal of extracellular vesicles · 2019Article
- Flow Virometry: a Powerful Tool To Functionally Characterize Viruses.Journal of virology · 2018Review
- Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines.Journal of extracellular vesicles · 2018Article
- Systematic Methodological Evaluation of a Multiplex Bead-Based Flow Cytometry Assay for Detection of Extracellular Vesicle Surface Signatures.Frontiers in immunology · 2018Article
- Single-Particle Discrimination of Retroviruses from Extracellular Vesicles by Nanoscale Flow Cytometry.Scientific reports · 2017Article
- The Methods of Choice for Extracellular Vesicles (EVs) Characterization.International journal of molecular sciences · 2017Review
- Detection of platelet vesicles by flow cytometry.Platelets · 2017Review
- Flow virometric sorting and analysis of HIV quasispecies from plasma.JCI insight · 2017Article
- Extracellular Vesicle Flow Cytometry Analysis and Standardization.Frontiers in cell and developmental biology · 2017Review
- Detecting individual extracellular vesicles using a multicolor in situ proximity ligation assay with flow cytometric readout.Scientific reports · 2016Article
- Article
- A Flow-Cytometry-Based Approach to Facilitate Quantification, Size Estimation and Characterization of Sub-visible Particles in Protein Solutions.Pharmaceutical research · 2015Article
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2 authors.
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Abstract
The analysis of individual nanoparticles by flow cytometry involves the measurement of dim signals that are near the detection limits of the instrument. Discriminating the signal from particles of interest from that of background particles in buffers and from optical and electronic noise can be challenging, and requires careful consideration of the measurement approach, control experiments, and scrutiny of the resulting data. In applying this scrutiny, we have come to recognize an artifact that results from the inappropriate selection of the trigger channel threshold that might not be obvious to the casual user. When measuring dim signals close to the noise or background levels, it is intuitive and common for the operator to adjust the trigger threshold to minimize the "false triggers" acquired by the system, and then to run the unknown sample, interpreting the events detected above the background as measurements of individual particles. We show here that when this approach is used to measure particles whose signals fall below the trigger threshold, only coincident events are detected, producing erroneous measurements of both particle number and brightness. We suggest that in many cases, the analysis of dim nanoparticles is best achieved using a fluorescence channel for the trigger.
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