ArticleInternational journal of molecular sciences2013
pH dependence of the fluorescence lifetime of FAD in solution and in cells.
Article in International journal of molecular sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed.
- Strategies and challenges in developing pre-vascularized three-dimensional skin substitutes using layer-by-layer cell coating technology.Regenerative therapy · 2026Article
- Fluorescence spectroscopy as a tool for differentiating methicillin resistance in clinical isolates of Staphylococcus aureus.Journal of fluorescence · 2026Article
- Real-time, high-resolution metabolic characterization of live bacteria using label-free optical metabolic imaging.NPJ biofilms and microbiomes · 2026Article
- Label-free fluorescence lifetime imaging can distinguish cancer from healthy tissue in spontaneously occurring canine oral tumors.Scientific reports · 2026Article
- Autofluorescence lifetime of gelatin-methacrylate hydrogels is sensitive to changes in crosslinking and post-gelation pH.Frontiers in bioengineering and biotechnology · 2026Article
- Phasor-based FLIM analysis of NAD(P)H and FAD autofluorescence for label-free bacterial classification.Journal of biomedical optics · 2026Article
- Non-canonical NOTCH1 signaling regulates ferroptosis vulnerability in dormant lung cancer cells with stable resistance.Cell death & disease · 2025Article
- Visualizing intracellular milieu and electric field-induced cell responses by fluorescence lifetime imaging microscopy.Scientific reports · 2025Article
- Fluorescence Spectroscopy Based Identification of Pseudomonas Aeruginosa and Escherichia Coli Suspensions.Journal of fluorescence · 2025Article
- Insights into metabolic changes during epidermal differentiation as revealed by multiphoton microscopy with fluorescence lifetime imaging.Scientific reports · 2025Article
- Quantum theory of a potential biological magnetic field sensor: Radical pair mechanism in flavin adenine dinucleotide biradicals.Computational and structural biotechnology journal · 2024Article
- Fast autofluorescence imaging to evaluate dynamic changes in cell metabolism.Journal of biomedical optics · 2024Article
- State-of-the-Art in Skin Fluorescent Photography for Cosmetic and Skincare Research: From Molecular Spectra to AI Image Analysis.Life (Basel, Switzerland) · 2024Review
- pH modulates efficiency of singlet oxygen production by flavin cofactors.RSC advances · 2024Article
- Fluorescence Spectroscopy Based Characterization of Pseudomonas Aeruginosa Suspension.Journal of fluorescence · 2024Article
- Antimicrobial photodynamic therapy against Escherichia coli by exploiting endogenously produced Protoporphyrin IX- In vitro study.Lasers in medical science · 2024Article
- Plastic vasomotion entrainment.eLife · 2024Article
- Autofluorescence is a biomarker of neural stem cell activation state.Cell stem cell · 2024Article
- Flavinated SDHA underlies the change in intrinsic optical properties of oral cancers.Communications biology · 2023Article
- The Dual Luminescence Lifetime pH/Oxygen Sensor: Evaluation of Applicability for Intravital Analysis of 2D- and 3D-Cultivated Human Endometrial Mesenchymal Stromal Cells.International journal of molecular sciences · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied physiological parameters in a living cell using fluorescence lifetime imaging of endogenous chromophores. In this study, pH dependence of the fluorescence lifetime of flavin adenine dinucleotide (FAD), that is a significant cofactor exhibiting autofluorescence, has been investigated in buffer solution and in cells. The fluorescence lifetime of FAD remained unchanged with pH 5 to 9 in solution. However, the fluorescence lifetime in HeLa cells was found to decrease with increasing intracellular pH, suggesting that pH in a single cell can be estimated from the fluorescence lifetime imaging of FAD without adding exogenous fluorescent probes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.