ArticleNature2013
A complete mass-spectrometric map of the yeast proteome applied to quantitative trait analysis.
Article in Nature, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 144 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
144 citing papers in PubMed.
- In-Cell Proteomics Enables High-Resolution Temporal Profiling of Cell Cycle Progression and DNA Damage Response in Saccharomyces cerevisiae.Proteomics · 2026Article
- Adaptive Modeling of Tandem Mass Spectrometry Data: Creation of the METLIN 960K MRM Database.Analytical chemistry · 2025Article
- A genome-to-proteome map reveals how natural variants drive proteome diversity and shape fitness.Science (New York, N.Y.) · 2025Article
- Spatial top-down proteomics for the functional characterization of human kidney.Clinical proteomics · 2025Article
- Understanding the molecular diversity of synapses.Nature reviews. Neuroscience · 2025Review
- A genome-to-proteome atlas charts natural variants controlling proteome diversity and forecasts their fitness effects.bioRxiv : the preprint server for biology · 2024Article
- Spatial top-down proteomics for the functional characterization of human kidney.bioRxiv : the preprint server for biology · 2024Article
- What are the 100 most cited fungal genera?Studies in mycology · 2024Article
- Quantitative Peptidomics: General Considerations.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Microanalytical Mass Spectrometry with Super-Resolution Microscopy Reveals a Proteome Transition During Development of the Brain's Circadian Pacemaker.Analytical chemistry · 2023Article
- Generalized precursor prediction boosts identification rates and accuracy in mass spectrometry based proteomics.Communications biology · 2023Article
- Prioritized mass spectrometry increases the depth, sensitivity and data completeness of single-cell proteomics.Nature methods · 2023Article
- Using SILAC to Develop Quantitative Data-Independent Acquisition (DIA) Proteomic Methods.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Proteome-wide systems genetics identifies UFMylation as a regulator of skeletal muscle function.eLife · 2022Article
- Review
- Proteotype coevolution and quantitative diversity across 11 mammalian species.Science advances · 2022Article
- Quantitative Characterisation of Low Abundant Yeast Mitochondrial Proteins Reveals Compensation for Haplo-Insufficiency in Different Environments.International journal of molecular sciences · 2022Article
- Mapping of promoter usage QTL using RNA-seq data reveals their contributions to complex traits.PLoS computational biology · 2022Article
- The impact of genomic variation on protein phosphorylation states and regulatory networks.Molecular systems biology · 2022Article
84 more citing papers are in PubMed but not listed here.
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19 authors.
Funding
Abstract
Experience from different fields of life sciences suggests that accessible, complete reference maps of the components of the system under study are highly beneficial research tools. Examples of such maps include libraries of the spectroscopic properties of molecules, or databases of drug structures in analytical or forensic chemistry. Such maps, and methods to navigate them, constitute reliable assays to probe any sample for the presence and amount of molecules contained in the map. So far, attempts to generate such maps for any proteome have failed to reach complete proteome coverage. Here we use a strategy based on high-throughput peptide synthesis and mass spectrometry to generate an almost complete reference map (97% of the genome-predicted proteins) of the Saccharomyces cerevisiae proteome. We generated two versions of this mass-spectrometric map, one supporting discovery-driven (shotgun) and the other supporting hypothesis-driven (targeted) proteomic measurements. Together, the two versions of the map constitute a complete set of proteomic assays to support most studies performed with contemporary proteomic technologies. To show the utility of the maps, we applied them to a protein quantitative trait locus (QTL) analysis, which requires precise measurement of the same set of peptides over a large number of samples. Protein measurements over 78 S. cerevisiae strains revealed a complex relationship between independent genetic loci, influencing the levels of related proteins. Our results suggest that selective pressure favours the acquisition of sets of polymorphisms that adapt protein levels but also maintain the stoichiometry of functionally related pathway members.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.