ArticleBiochemical and biophysical research communications2013
WD40-repeat protein WDR18 collaborates with TopBP1 to facilitate DNA damage checkpoint signaling.
Article in Biochemical and biophysical research communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- An atlas of the shared genetic architecture between atopic and gastrointestinal diseases.Communications biology · 2024Article
- Distinct regulation of ATM signaling by DNA single-strand breaks and APE1.Nature communications · 2024Article
- Structure-function analysis of TOPBP1's role in ATR signaling using the DSB-mediated ATR activation in Xenopus egg extracts (DMAX) system.Scientific reports · 2021Article
- APE2 Is a General Regulator of the ATR-Chk1 DNA Damage Response Pathway to Maintain Genome Integrity in Pancreatic Cancer Cells.Frontiers in cell and developmental biology · 2021Article
- Methods for Studying DNA Single-Strand Break Repair and Signaling in Xenopus laevis Egg Extracts.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Single-Strand Break End Resection in Genome Integrity: Mechanism and Regulation by APE2.International journal of molecular sciences · 2018Review
- Cell-free Xenopus egg extracts for studying DNA damage response pathways.The International journal of developmental biology · 2016Review
- REV1 is important for the ATR-Chk1 DNA damage response pathway in Xenopus egg extracts.Biochemical and biophysical research communications · 2015Article
- A physical association between the human mutY homolog (hMYH) and DNA topoisomerase II-binding protein 1 (hTopBP1) regulates Chk1-induced cell cycle arrest in HEK293 cells.Cell & bioscience · 2015Article
- The Multifunctions of WD40 Proteins in Genome Integrity and Cell Cycle Progression.Journal of genomics · 2015Review
- Functional interplay between ATM/ATR-mediated DNA damage response and DNA repair pathways in oxidative stress.Cellular and molecular life sciences : CMLS · 2014Review
- Importin β-dependent nuclear import of TopBP1 in ATR-Chk1 checkpoint in Xenopus egg extracts.Cellular signalling · 2014Article
- APE2 is required for ATR-Chk1 checkpoint activation in response to oxidative stress.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The genomes of all living organisms are exposed to a wide spectrum of insults. To maintain genomic integrity, eukaryotes have evolved an elaborate surveillance mechanism - DNA damage checkpoint signaling - to detect damaged DNA and to arrest cell cycle progression, allowing time to process and repair DNA damage. TopBP1 plays multiple roles in the regulation of DNA damage checkpoint signaling. However, the molecular mechanism of how TopBP1 regulates ATR-mediated Chk1 phosphorylation is poorly understood. In this communication, we demonstrate (1) that the Chk1 activation domain of TopBP1 is critical in response to several different types of DNA damage; (2) that WD40-repeat protein WDR18 associates with the C-terminus of TopBP1 in vitro and in vivo; (3) that the association between WDR18 and TopBP1 is required for AT70-induced Chk1 phosphorylation; (4) and that WDR18 itself is required for AT70-triggered Chk1 phosphorylation. In addition, WDR18 associates with Chk1 in vitro. The data suggest that WDR18 facilitates ATR-dependent Chk1 phosphorylation via interacting with both C-terminus of TopBP1 and Chk1. Our findings indicate that WDR18 is a bona fide checkpoint protein and that WDR18 works together with TopBP1 to promote DNA damage checkpoint signaling.
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