ArticleMicrobial biotechnology2013
Integrative modelling of pH-dependent enzyme activity and transcriptomic regulation of the acetone-butanol-ethanol fermentation of Clostridium acetobutylicum in continuous culture.
Article in Microbial biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- In situ buffering and enzymatic regulation of acidogenesis in two-stage anaerobic digestion of composite vegetable waste.Scientific reports · 2026Article
- Exploring the Influence of pH on the Dynamics of Acetone-Butanol-Ethanol Fermentation.Microorganisms · 2023Article
- pH dependencies of glycolytic enzymes of yeast under in vivo-like assay conditions.The FEBS journal · 2022Article
- Kinetic Modeling ofMetabolites · 2022Review
- Crystal Structure and Molecular Mechanism of Phosphotransbutyrylase fromJournal of microbiology and biotechnology · 2021Article
- Protein Acetylation and Butyrylation Regulate the Phenotype and Metabolic Shifts of the Endospore-formingMolecular & cellular proteomics : MCP · 2018Article
- Remarkable reactivity of alkoxide/acetato-bridged binuclear copper(II) complex as artificial carboxylesterase.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2017Article
- Mathematical modelling of clostridial acetone-butanol-ethanol fermentation.Applied microbiology and biotechnology · 2017Review
- Article
- Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum.Nature communications · 2015Article
- Gene duplication and the environmental regulation of physiology and development.Ecology and evolution · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In a continuous culture under phosphate limitation the metabolism of Clostridium acetobutylicum depends on the external pH level. By comparing seven steady-state conditions between pH 5.7 and pH 4.5 we show that the switch from acidogenesis to solventogenesis occurs between pH 5.3 and pH 5.0 with an intermediate state at pH 5.1. Here, an integrative study is presented investigating how a changing external pH level affects the clostridial acetone-butanol-ethanol (ABE) fermentation pathway. This is of particular interest as the biotechnological production of n-butanol as biofuel has recently returned into the focus of industrial applications. One prerequisite is the furthering of the knowledge of the factors determining the solvent production and their integrative regulations. We have mathematically analysed the influence of pH-dependent specific enzyme activities of branch points of the metabolism on the product formation. This kinetic regulation was compared with transcriptomic regulation regarding gene transcription and the proteomic profile. Furthermore, both regulatory mechanisms were combined yielding a detailed projection of their individual and joint effects on the product formation. The resulting model represents an important platform for future developments of industrial butanol production based on C. acetobutylicum.
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