ArticleVeterinary research2013
Identification of Dirofilaria immitis miRNA using illumina deep sequencing.
Article in Veterinary research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Development of Dirofilaria immitis adult worms in NSG mice, detection of parasite-derived microRNA and comparative analysis of laboratory isolates.Scientific reports · 2026Article
- Article
- The expanding role of microRNAs in the biology and control of veterinary parasitic nematodes.Frontiers in veterinary science · 2025Review
- Evaluation of diagnostic techniques for early detection of heartworm in experimentally infected dogs: identification of Dirofilaria immitis-derived microRNA in the initial 28 weeks post-inoculation.Parasites & vectors · 2024Article
- Secretory microRNA Profiles of Third- and Fourth-StagePathogens (Basel, Switzerland) · 2021Article
- Small RNAs in parasitic nematodes - forms and functions.Parasitology · 2020Review
- Identification and characterization of microRNAs in the pituitary of pubescent goats.Reproductive biology and endocrinology : RB&E · 2018Article
- Perusal of parasitic nematode 'omics in the post-genomic era.Molecular and biochemical parasitology · 2017Review
- Plasma-derived parasitic microRNAs have insufficient concentrations to be used as diagnostic biomarker for detection of Onchocerca volvulus infection or treatment monitoring using LNA-based RT-qPCR.Parasitology research · 2017Article
- Evaluation of the diagnostic potential of urinary N-Acetyltyramine-O,β-glucuronide (NATOG) as diagnostic biomarker for Onchocerca volvulus infection.Parasites & vectors · 2016Article
- MicroRNA profiling of antler stem cells in potentiated and dormant states and their potential roles in antler regeneration.Molecular genetics and genomics : MGG · 2016Article
- Functional analysis of microRNA activity in Brugia malayi.International journal for parasitology · 2015Article
- A novel member of the let-7 microRNA family is associated with developmental transitions in filarial nematode parasites.BMC genomics · 2015Article
- Detection of circulating parasite-derived microRNAs in filarial infections.PLoS neglected tropical diseases · 2014Article
- Intraspecific genetic variation and phylogenetic analysis of Dirofilaria immitis samples from Western China using complete ND1 and 16S rDNA gene sequences.The American journal of tropical medicine and hygiene · 2014Article
- Comparative analysis of microRNA profiles between adult Ascaris lumbricoides and Ascaris suum.BMC veterinary research · 2014Article
- Nematode endogenous small RNA pathways.Worm · 2014Review
- Identification of neglected cestode Taenia multiceps microRNAs by illumina sequencing and bioinformatic analysis.BMC veterinary research · 2013Article
- Functional diversification of Argonautes in nematodes: an expanding universe.Biochemical Society transactions · 2013Review
Corrections and comments
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Authors and funding
19 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The heartworm Dirofilaria immitis is the causative agent of cardiopulmonary dirofilariosis in dogs and cats, which also infects a wide range of wild mammals and humans. The complex life cycle of D. immitis with several developmental stages in its invertebrate mosquito vectors and its vertebrate hosts indicates the importance of miRNA in growth and development, and their ability to regulate infection of mammalian hosts. This study identified the miRNA profiles of D. immitis of zoonotic significance by deep sequencing. A total of 1063 conserved miRNA candidates, including 68 anti-sense miRNA (miRNA*) sequences, were predicted by computational methods and could be grouped into 808 miRNA families. A significant bias towards family members, family abundance and sequence nucleotides was observed. Thirteen novel miRNA candidates were predicted by alignment with the Brugia malayi genome. Eleven out of 13 predicted miRNA candidates were verified by using a PCR-based method. Target genes of the novel miRNA candidates were predicted by using the heartworm transcriptome dataset. To our knowledge, this is the first report of miRNA profiles in D. immitis, which will contribute to a better understanding of the complex biology of this zoonotic filarial nematode and the molecular regulation roles of miRNA involved. Our findings may also become a useful resource for small RNA studies in other filarial parasitic nematodes.
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Registered trials
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