ArticleInsect molecular biology2013
Transgene-mediated suppression of the RNA interference pathway in Aedes aegypti interferes with gene silencing and enhances Sindbis virus and dengue virus type 2 replication.
Article in Insect molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Optimizing CRE and PhiC31 mediated recombination inFrontiers in bioengineering and biotechnology · 2023Article
- Understanding Molecular Pathogenesis with Chikungunya Virus Research Tools.Current topics in microbiology and immunology · 2022Review
- Delineating the Role ofPathogens (Basel, Switzerland) · 2021Article
- Distinct Roles of Hemocytes at Different Stages of Infection by Dengue and Zika Viruses inFrontiers in immunology · 2021Article
- Using Diverse Model Systems to Define Intestinal Epithelial Defenses to Enteric Viral Infections.Cell host & microbe · 2020Review
- Arboviruses and the Challenge to Establish Systemic and Persistent Infections in Competent Mosquito Vectors: The Interaction With the RNAi Mechanism.Frontiers in physiology · 2019Review
- The small non-coding RNA response to virus infection in the Leishmania vector Lutzomyia longipalpis.PLoS neglected tropical diseases · 2018Article
- Individual co-variation between viral RNA load and gene expression reveals novel host factors during early dengue virus infection of the Aedes aegypti midgut.PLoS neglected tropical diseases · 2017Article
- Review
- Small Interfering RNA Pathway Modulates Initial Viral Infection in Midgut Epithelium of Insect after Ingestion of Virus.Journal of virology · 2016Article
- Flavivirus sfRNA suppresses antiviral RNA interference in cultured cells and mosquitoes and directly interacts with the RNAi machinery.Virology · 2015Article
- Tissue Barriers to Arbovirus Infection in Mosquitoes.Viruses · 2015Review
- Disruption of dengue virus transmission by mosquitoes.Current opinion in insect science · 2015Article
- Fighting Arbovirus Transmission: Natural and Engineered Control of Vector Competence in Aedes Mosquitoes.Insects · 2015Review
- Rapid selection against arbovirus-induced apoptosis during infection of a mosquito vector.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Antiviral immunity of Anopheles gambiae is highly compartmentalized, with distinct roles for RNA interference and gut microbiota.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Heritable CRISPR/Cas9-mediated genome editing in the yellow fever mosquito, Aedes aegypti.PloS one · 2015Article
- Suppressing dengue-2 infection by chemical inhibition of Aedes aegypti host factors.PLoS neglected tropical diseases · 2014Article
- Fitness impact and stability of a transgene conferring resistance to dengue-2 virus following introgression into a genetically diverse Aedes aegypti strain.PLoS neglected tropical diseases · 2014Article
- Novel Genetic and Molecular Tools for the Investigation and Control of Dengue Virus Transmission by Mosquitoes.Current tropical medicine reports · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
RNA interference (RNAi) is the major innate antiviral pathway in Aedes aegypti that responds to replicating arboviruses such as dengue virus (DENV) and Sindbis virus (SINV). On the one hand, the mosquito's RNAi machinery is capable of completely eliminating DENV2 from Ae. aegypti. On the other, transient silencing of key genes of the RNAi pathway increases replication of SINV and DENV2, allowing the viruses to temporally overcome dose-dependent midgut infection and midgut escape barriers (MEB) more efficiently. Here we expressed Flock house virus B2 (FHV-B2) from the poly-ubiquitin (PUb) promoter in Ae. aegypti using the ΦC31 site-directed recombination system to investigate the impact of transgene-mediated RNAi pathway suppression on infections with SINV-TR339eGFP and DENV2-QR94, the latter of which has been shown to be confronted with a strong MEB in Ae. aegypti. FHV-B2 was constitutively expressed in midguts of sugar- and blood-fed mosquitoes of transgenic line PUbB2 P61. B2 over-expression suppressed RNA silencing of carboxypeptidase A-1 (AeCPA-1) in midgut tissue of PUbB2 P61 mosquitoes. Following oral challenge with SINV-TR339eGFP or DENV2-QR94, mean titres in midguts of PUbB2 P61 females were significantly higher at 7 days post-bloodmeal (pbm) than in those of nontransgenic control mosquitoes. At 14 days pbm, infection rates of carcasses were significantly increased in PubB2 P61 mosquitoes infected with SINV-TR339eGFP. Following infection with DENV2-QR94, midgut infection rates were significantly increased in the B2-expressing mosquitoes at 14 days pbm. However, B2 expression in PUbB2 P61 did not increase the DENV2-QR94 dissemination rate, indicating that the infection phenotype was not primarily controlled by RNAi.
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