ArticleAnalytical chemistry2013
Elastomeric negative acoustic contrast particles for affinity capture assays.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Acoustic pipette and biofunctional elastomeric microparticle system for rapid picomolar-level biomolecule detection in whole blood.Science advances · 2024Article
- Essential Fluidics for a Flow Cytometer.Current protocols · 2024Article
- Biomolecular actuators for genetically selective acoustic manipulation of cells.Science advances · 2023Article
- Two-Step Acoustophoresis Separation of Live Tumor Cells from Whole Blood.Analytical chemistry · 2021Article
- Directed Assembly of Particles for Additive Manufacturing of Particle-Polymer Composites.Micromachines · 2021Review
- Antibody Conjugate Assembly on Ultrasound-Confined Microcarrier Particles.ACS biomaterials science & engineering · 2020Article
- Ultrasound Standing Wave-Based Cell-to-liquid Separation for Measuring Viscosity and Aggregation of Blood Sample.Sensors (Basel, Switzerland) · 2020Article
- Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches.International journal of molecular sciences · 2020Review
- Reducing WBC background in cancer cell separation products by negative acoustic contrast particle immuno-acoustophoresis.Analytica chimica acta · 2018Article
- Rapid and effective enrichment of mononuclear cells from blood using acoustophoresis.Scientific reports · 2017Article
- MicroBubble activated acoustic cell sorting.Biomedical microdevices · 2017Article
- Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles.Journal of visualized experiments : JoVE · 2016Article
- Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation.Lab on a chip · 2015Review
- Two-dimensional spatial manipulation of microparticles in continuous flows in acoustofluidic systems.Biomicrofluidics · 2015Article
- The intersection of flow cytometry with microfluidics and microfabrication.Lab on a chip · 2014Review
- Separation of Escherichia coli bacteria from peripheral blood mononuclear cells using standing surface acoustic waves.Analytical chemistry · 2013Article
- Fluidics.Current protocols in cytometry · 2013Article
- Elastomeric microparticles for acoustic mediated bioseparations.Journal of nanobiotechnology · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
This report describes the development of elastomeric capture microparticles (ECμPs) and their use with acoustophoretic separation to perform microparticle assays via flow cytometry.We have developed simple methods to form ECμPs by cross-linking droplets of common commercially available silicone precursors in suspension followed by surface functionalization with biomolecular recognition reagents. The ECμPs are compressible particles that exhibit negative acoustic contrast in ultrasound when suspended in aqueous media, blood serum, or diluted blood. In this study, these particles have been functionalized with antibodies to bind prostate specific antigen and immunoglobulin (IgG). Specific separation of the ECμPs from blood cells is achieved by flowing them through a microfluidic acoustophoretic device that uses an ultrasonic standing wave to align the blood cells, which exhibit positive acoustic contrast, at a node in the acoustic pressure distribution while aligning the negative acoustic contrast ECμPs at the antinodes. Laminar flow of the separated particles to downstream collection ports allows for collection of the separated negative contrast (ECμPs) and positive contrast particles (cells). Separated ECμPs were analyzed via flow cytometry to demonstrate nanomolar detection for prostate specific antigen in aqueous buffer and picomolar detection for IgG in plasma and diluted blood samples. This approach has potential applications in the development of rapid assays that detect the presence of low concentrations of biomarkers in a number of biological sample types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.