ArticleThe Journal of biological chemistry2013
Regulation of Argonaute slicer activity by guide RNA 3' end interactions with the N-terminal lobe.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 45 citations in OpenAlex.
- Dynamic monomer-dimer transition regulates DNA-guided RNA cleavage by MbpAgo.Nature communications · 2026Article
- C-terminal tagging impairs AGO2 function.RNA biology · 2025Article
- The structural basis for RNA slicing by human Argonaute2.Cell reports · 2025Article
- The structural basis for RNA slicing by human Argonaute2.bioRxiv : the preprint server for biology · 2024Article
- Target DNA-dependent activation mechanism of the prokaryotic immune system SPARTA.Nucleic acids research · 2024Article
- Non-destructive and efficient method for obtaining miRNA information in cells by artificial extracellular vesicles.Scientific reports · 2023Article
- Research progress in mitochondrial gene editing technology.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2023Article
- Argonaute protein-based nucleic acid detection technology.Frontiers in microbiology · 2023Review
- Single-molecule FRET uncovers hidden conformations and dynamics of human Argonaute 2.Nature communications · 2022Article
- The Characters of Non-Coding RNAs and Their Biological Roles in Plant Development and Abiotic Stress Response.International journal of molecular sciences · 2022Review
- Prokaryotic Argonaute Proteins as a Tool for Biotechnology.Molecular biology · 2022Article
- NgAgo possesses guided DNA nicking activity.Nucleic acids research · 2021Article
- Argonaute proteins: structures and their endonuclease activity.Molecular biology reports · 2021Review
- Mechanisms of Novel Host Use by Bactrocera tau (Tephritid: Diptera) Revealed by RNA Transcriptomes.Journal of insect science (Online) · 2020Article
- WheatFrontiers in plant science · 2020Article
- AGO unchained: Canonical and non-canonical roles of Argonaute proteins in mammals.Frontiers in bioscience (Landmark edition) · 2020Review
- Structural Basis for Target-Directed MicroRNA Degradation.Molecular cell · 2019Article
- Distinct roles of Argonaute in the green alga Chlamydomonas reveal evolutionary conserved mode of miRNA-mediated gene expression.Scientific reports · 2019Article
- DNA interference and beyond: structure and functions of prokaryotic Argonaute proteins.Nature communications · 2018Review
- Structural and functional analyses reveal the contributions of the C- and N-lobes of Argonaute protein to selectivity of RNA target cleavage.The Journal of biological chemistry · 2018Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Structural studies indicate that binding of both the guide RNA (siRNA and miRNA) and the target mRNA trigger substantial conformational changes in the Argonaute proteins. Here we explore the role of the N-terminal lobe (and its PAZ domain) in these conformational changes using biochemical and cell culture-based approaches. In vitro, whereas deletion (or mutation) of the N-terminal lobe of DmAgo1 and DmAgo2 had no effect on binding affinity to guide RNAs, we observed a loss of protection of the 3' end of the guide RNA and decreased target RNA binding; consistent with this, in cells, loss of function DmAgo1 PAZ variant proteins (PAZ6 and ΔN-PAZ) still bind RNA, although the RNAs are shorter than normal. We also find that deletion of the N-terminal lobe results in constitutive activation of endogenous PIWI domain-based cleavage activity in vitro, providing insights into how cleavage activity may be regulated in vivo in response to different types of pairing interactions with the target mRNAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.