ArticleMicrobiology (Reading, England)2013
Genetic and chemical characterization of ibuprofen degradation by Sphingomonas Ibu-2.
Article in Microbiology (Reading, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 97 citations in OpenAlex.
- A pathway for complete mineralization of the ambergris constituent (-)-Ambrox inApplied and environmental microbiology · 2026Article
- Molecular Monitoring in Soil Bioremediation: From Genetic Potential to Verified Pathway Operation.International journal of molecular sciences · 2026Review
- Removal of Ibuprofen from Contaminated Water by Bioaugmentation with Novel Bacterial Strains Isolated from Sewage Sludge.Microorganisms · 2025Article
- Transformation of the drug ibuprofen by Priestia megaterium: reversible glycosylation and generation of hydroxylated metabolites.Environmental science and pollution research international · 2025Article
- Genetic and species rearrangements in microbial consortia impact biodegradation potential.The ISME journal · 2025Article
- Removal of Ibuprofen in Water by Bioaugmentation withJournal of xenobiotics · 2024Article
- New bacterial strains for ibuprofen biodegradation: Drug removal, transformation, and potential catabolic genes.Environmental microbiology reports · 2024Article
- Improving the production of 22-hydroxy-23,24-bisnorchol-4-ene-3-one in Mycolicibacterium smegmatis.Microbial biotechnology · 2024Article
- Pollutant profile complexity governs wastewater removal of recalcitrant pharmaceuticals.The ISME journal · 2024Article
- Microbial degradation of contaminants of emerging concern: metabolic, genetic and omics insights for enhanced bioremediation.Frontiers in bioengineering and biotechnology · 2024Review
- Analyzing microbial communities and their biodegradation of multiple pharmaceuticals in membrane bioreactors.Applied microbiology and biotechnology · 2023Article
- The Impact of Anti-Inflammatory Drugs on the Prokaryotic Community Composition and Selected Bacterial Strains Based on Microcosm Experiments.Microorganisms · 2023Article
- Ibuprofen: Toxicology and Biodegradation of an Emerging Contaminant.Molecules (Basel, Switzerland) · 2023Review
- Article
- Diclofenac, ibuprofen, and paracetamol biodegradation: overconsumed non-steroidal anti-inflammatories drugs at COVID-19 pandemic.Frontiers in microbiology · 2023Review
- Characteristics and growth kinetics of biomass of Citrobacter freundii strains PYI-2 and Citrobacter portucalensis strain YPI-2 during the biodegradation of Ibuprofen.International microbiology : the official journal of the Spanish Society for Microbiology · 2022Article
- Genetic Characterization of the Ibuprofen-Degradative Pathway of Rhizorhabdus wittichii MPO218.Applied and environmental microbiology · 2022Article
- Engineering Saccharomyces cerevisiae for production of the capsaicinoid nonivamide.Microbial cell factories · 2022Article
- Clover Root Exudates FavormSphere · 2021Article
- Ibuprofen Degradation and Associated Bacterial Communities in Hyporheic Zone Sediments.Microorganisms · 2020Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Sphingomonas Ibu-2 has the unusual ability to cleave the acid side chain from the pharmaceutical ibuprofen and related arylacetic acid derivatives to yield corresponding catechols under aerobic conditions via a previously uncharacterized mechanism. Screening a chromosomal library of Ibu-2 DNA in Escherichia coli EPI300 allowed us to identify one fosmid clone (pFOS3G7) that conferred the ability to metabolize ibuprofen to isobutylcatechol. Characterization of pFOS3G7 loss-of-function transposon mutants permitted identification of five ORFs, ipfABDEF, whose predicted amino acid sequences bore similarity to the large and small units of an aromatic dioxygenase (ipfAB), a sterol carrier protein X (SCPx) thiolase (ipfD), a domain of unknown function 35 (DUF35) protein (ipfE) and an aromatic CoA ligase (ipfF). Two additional ORFs, ipfH and ipfI, which encode putative ferredoxin reductase and ferredoxin components of an aromatic dioxygenase system, respectively, were also identified on pFOS3G7. Complementation of a markerless loss-of-function ipfD deletion mutant restored catechol production as did complementation of the ipfF Tn mutant. Expression of subcloned ipfABDEF alone in E. coli did not impart full metabolic activity unless coexpressed with ipfHI. CoA ligation followed by ring oxidation is common to phenylacetic acid pathways. However, the need for a putative SCPx thiolase (IpfD) and DUF35 protein (IpfE) in aerobic arylacetic acid degradation is unprecedented. This work provides preliminary insights into the mechanism behind this novel arylacetic acid-deacylating, catechol-generating activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.