ArticleMicrobiology (Reading, England)2013
A(2) expression and assembly regulates lysis in Qβ infections.
Article in Microbiology (Reading, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Carboxyl-Terminal Region of the Maturation Protein of RNA Coliphage Qβ Mediates Host Attachment, Virion Formation, and Cell Lysis.Genes to cells : devoted to molecular & cellular mechanisms · 2026Article
- Phage lysis protein LysScience advances · 2025Article
- Physiological characterization of single-gene lysis proteins.Journal of bacteriology · 2024Article
- Physiological characterization of single gene lysis proteins.bioRxiv : the preprint server for biology · 2023Article
- Review
- Review
- The balance between fitness advantages and costs drives adaptation of bacteriophage Qβ to changes in host density at different temperatures.Frontiers in microbiology · 2023Article
- Article
- Phage display as a tool for vaccine and immunotherapy development.Bioengineering & translational medicine · 2020Review
- Phage single-gene lysis: Finding the weak spot in the bacterial cell wall.The Journal of biological chemistry · 2019Review
- Phage Lysis: Multiple Genes for Multiple Barriers.Advances in virus research · 2019Review
- RNA Phage Biology in a Metagenomic Era.Viruses · 2018Review
- Expression of a Peptidoglycan Hydrolase from Lytic Bacteriophages Atu_ph02 and Atu_ph03 Triggers Lysis of Agrobacterium tumefaciens.Applied and environmental microbiology · 2017Article
- Structures of Qβ virions, virus-like particles, and the Qβ-MurA complex reveal internal coat proteins and the mechanism of host lysis.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Asymmetric cryo-EM structure of the canonical Allolevivirus Qβ reveals a single maturation protein and the genomic ssRNA in situ.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Bacteriophages and phage-derived proteins--application approaches.Current medicinal chemistry · 2015Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The capsids of ssRNA phages comprise a single copy of an ~45 kDa maturation protein that serves to recognize the conjugative pilus as receptor, to protect the ends of the viral RNA and also to escort the genomic RNA into the host cytoplasm. In the Alloleviviridae, represented by the canonical phage Qβ, the maturation protein A(2) also causes lysis. This is achieved by inhibiting the activity of MurA, which catalyses the first committed step of murein biosynthesis. Previously, it was shown that Qβ virions, with a single copy of A(2), inhibit MurA activity. This led to a model for lysis timing in which, during phage infection, A(2) is not active as a MurA inhibitor until assembled into virion particles, thus preventing premature lysis before a sufficient yield of viable progeny has accumulated. Here we report that MurA inactivates purified Qβ particles, casting doubt on the notion that A(2) must assemble into particles prior to MurA inhibition. Furthermore, quantification of A(2) protein induced from a plasmid indicated that lysis is entrained when the amount of the lysis protein is approximately equimolar to that of cellular MurA. Qβ por mutants, isolated as suppressors that overcome a murA(rat) mutation that reduces the affinity of MurA for A(2), were shown to be missense mutations in A(2) that increase the translation of the maturation protein. Because of the increased production of A(2), the por mutants have an attenuated infection cycle and reduced burst size, indicating that a delicate balance between assembled and unassembled A(2) levels regulates lysis timing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.