ArticleStem cells and development2013
MicroRNA-122 influences the development of sperm abnormalities from human induced pluripotent stem cells by regulating TNP2 expression.
Article in Stem cells and development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 54 citations in OpenAlex.
- Sperm abnormality: Differential expression of microRNAs.Journal of assisted reproduction and genetics · 2025Review
- Extrahepatic and Circulating miR-122: Diagnostic Implications and Future Directions.MicroRNA (Shariqah, United Arab Emirates) · 2025Review
- MicroRNA alterations in sperm of infertile men: Insights into oligozoospermia, asthenozoospermia, and teratozoospermia.Biotechnology notes (Amsterdam, Netherlands) · 2025Article
- Elucidating the Role of OXPHOS Variants in Asthenozoospermia: Insights from Whole Genome Sequencing and an In Silico Analysis.International journal of molecular sciences · 2024Article
- MicroRNAs in spermatogenesis dysfunction and male infertility: clinical phenotypes, mechanisms and potential diagnostic biomarkers.Frontiers in endocrinology · 2024Review
- Correlation between Sperm Micro Ribonucleic Acid-34b and -34c Levels and Clinical Outcomes of Intracytoplasmic Sperm Injection in Men with Male Factor Infertility.International journal of molecular sciences · 2022Article
- Overexpression ofInternational journal of reproductive biomedicine · 2022Article
- Comparison of miRNA and mRNA Expression in Sika Deer Testes With Age.Frontiers in veterinary science · 2022Article
- The epigenome of male germ cells and the programming of phenotypes in cattle.Animal frontiers : the review magazine of animal agriculture · 2021Article
- Coding and Non-Coding RNAs, as Male Fertility and Infertility Biomarkers.International journal of fertility & sterility · 2021Review
- A systems biology framework integrating GWAS and RNA-seq to shed light on the molecular basis of sperm quality in swine.Genetics, selection, evolution : GSE · 2020Article
- Distinct expression patterns of seven crucial microRNAs during early embryonic development in medaka (Oryzias latipes).Gene expression patterns : GEP · 2020Article
- Seminal plasma miRNAs in Klinefelter syndrome and in obstructive and non-obstructive azoospermia.Molecular biology reports · 2020Article
- miR-544 promotes maturity and antioxidation of stem cell-derived endothelial like cells by regulating the YY1/TET2 signalling axis.Cell communication and signaling : CCS · 2020Article
- Exploration of targets regulated by miR-125b in porcine adipocytes.In vitro cellular & developmental biology. Animal · 2020Article
- Downregulation of miR-322 promotes apoptosis of GC-2 cell by targeting Ddx3x.Reproductive biology and endocrinology : RB&E · 2019Article
- The role of microRNAs in embryonic stem cell and induced pluripotent stem cell differentiation in male germ cells.Journal of cellular physiology · 2019Review
- Differentially expressed microRNAs between cattleyak and yak testis.Scientific reports · 2018Article
- CHIR99021 combined with retinoic acid promotes the differentiation of primordial germ cells from human embryonic stem cells.Oncotarget · 2017Article
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Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sperm abnormalities are one of the main factors responsible for male infertility; however, their pathogenesis remains unclear. The role of microRNAs in the development of sperm abnormalities in infertile men has not yet been investigated. Here, we used human induced pluripotent stem cells to investigate the influence of miR-122 expression on the differentiation of these cells into spermatozoa-like cells in vitro. After induction, mutant miR-122-transfected cells formed spermatozoa-like cells. Flow cytometry of DNA content revealed a significant increase in the haploid cell population in spermatozoa-like cells derived from mutant miR-122-transfected cells as compared to those derived from miR-122-transfected cells. During induction, TNP2 and protamine mRNA and protein levels were significantly higher in mutant miR-122-transfected cells than in miR-122-transfected cells. High-throughput isobaric tags for relative and absolute quantification were used to identify and quantify the different protein expression levels in miR-122- and mutant miR-122-transfected cells. Among all the proteins analyzed, the expression of lipoproteins, for example, APOB and APOA1, showed the most significant difference between the two groups. This study illustrates that miR-122 expression is associated with abnormal sperm development. MiR-122 may influence spermatozoa-like cells by suppressing TNP2 expression and inhibiting the expression of proteins associated with sperm development.
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