ArticlePloS one2013
Adenosine stimulates the migration of human endothelial progenitor cells. Role of CXCR4 and microRNA-150.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- No Differences in Levels of Circulating Progenitor Endothelial Cells or Circulating Endothelial Cells Among Patients Treated With Ticagrelor Compared With Clopidogrel During Non- ST -Segment-Elevation Myocardial Infarction.Journal of the American Heart Association · 2018Trial
- Adenosine deaminase co-immunization reverses age-associated immunosenescence by restoring germinal center T follicular helper cell function.bioRxiv : the preprint server for biology · 2026Article
- MicroRNA: Crucial modulator in purinergic signalling involved diseases.Purinergic signalling · 2023Review
- Current Adenosinergic Therapies: What Do Cancer Cells Stand to Gain and Lose?International journal of molecular sciences · 2021Review
- Differential effect of adenosine on rhabdomyosarcoma migration and proliferation.Archives of medical science : AMS · 2020Article
- MicroRNA-221 Is Cardioprotective and Anti-fibrotic in a Rat Model of Myocardial Infarction.Molecular therapy. Nucleic acids · 2019Article
- MicroRNA 150-5p Improves Risk Classification for Mortality within 90 Days after Acute Ischemic Stroke.Journal of stroke · 2017Article
- Lung injury pathways: Adenosine receptor 2B signaling limits development of ischemic bronchiolitis obliterans organizing pneumonia.Experimental lung research · 2017Article
- A comparison of microRNA expression profiles from splenic hemangiosarcoma, splenic nodular hyperplasia, and normal spleens of dogs.BMC veterinary research · 2016Article
- MiR-150 Regulates Poststroke Cerebral Angiogenesis via Vascular Endothelial Growth Factor in Rats.CNS neuroscience & therapeutics · 2016Article
- Endothelial Progenitor Cells Physiology and Metabolic Plasticity in Brain Angiogenesis and Blood-Brain Barrier Modeling.Frontiers in physiology · 2016Review
- MicroRNAs; easy and potent targets in optimizing therapeutic methods in reparative angiogenesis.Journal of cellular and molecular medicine · 2015Review
- miR-150 Deficiency Protects against FAS-Induced Acute Liver Injury in Mice through Regulation of AKT.PloS one · 2015Article
- Period 2 is essential to maintain early endothelial progenitor cell function in vitro and angiogenesis after myocardial infarction in mice.Journal of cellular and molecular medicine · 2014Article
- MicroRNA regulation of bovine monocyte inflammatory and metabolic networks in an in vivo infection model.G3 (Bethesda, Md.) · 2014Article
- Article
- Impaired adenosine-mediated angiogenesis in preeclampsia: potential implications for fetal programming.Frontiers in pharmacology · 2014Review
- Effects of adenosine on lymphangiogenesis.PloS one · 2014Article
- Cardioprotective effects of adenosine within the border and remote areas of myocardial infarction.EJNMMI research · 2013Article
- Signal transduction in the development of pulmonary arterial hypertension.Pulmonary circulation · 2013Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAdministration of endothelial progenitor cells (EPC) represents a promising option to regenerate the heart after myocardial infarction, but is limited because of low recruitment and engraftment in the myocardium. Mobilization and migration of EPC are mainly controlled by stromal cell-derived factor 1α (SDF-1α) and its receptor CXCR4. We hypothesized that adenosine, a cardioprotective molecule, may improve the recruitment of EPC to the heart.
methodsEPC were obtained from peripheral blood mononuclear cells of healthy volunteers. Expression of chemokines and their receptors was evaluated using microarrays, quantitative PCR, and flow cytometry. A Boyden chamber assay was used to assess chemotaxis. Recruitment of EPC to the infarcted heart was evaluated in rats after permanent occlusion of the left anterior descending coronary artery.
resultsMicroarray analysis revealed that adenosine modulates the expression of several members of the chemokine family in EPC. Among these, CXCR4 was up-regulated by adenosine, and this result was confirmed by quantitative PCR (3-fold increase, P<0.001). CXCR4 expression at the cell surface was also increased. This effect involved the A(2B) receptor. Pretreatment of EPC with adenosine amplified their migration towards recombinant SDF-1α or conditioned medium from cardiac fibroblasts. Both effects were abolished by CXCR4 blocking antibodies. Adenosine also increased CXCR4 under ischemic conditions, and decreased miR-150 expression. Binding of miR-150 to the 3' untranslated region of CXCR4 was verified by luciferase assay. Addition of pre-miR-150 blunted the effect of adenosine on CXCR4. Administration of adenosine to rats after induction of myocardial infarction stimulated EPC recruitment to the heart and enhanced angiogenesis.
conclusionAdenosine increases the migration of EPC. The mechanism involves A(2B) receptor activation, decreased expression of miR-150 and increased expression of CXCR4. These results suggest that adenosine may be used to enhance the capacity of EPC to revascularize the ischemic heart.
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