ArticlePloS one2013
Variability of inducible expression across the hematopoietic system of tetracycline transactivator transgenic mice.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
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- Optimization of tamoxifen-induced Cre activity and its effect on immune cell populations.Scientific reports · 2020Article
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- Remethylation ofProceedings of the National Academy of Sciences of the United States of America · 2020Article
- A Mitochondrial Progesterone Receptor Increases Cardiac Beta-Oxidation and Remodeling.Journal of the Endocrine Society · 2019Article
- DNA-binding of the Tet-transactivator curtails antigen-induced lymphocyte activation in mice.Nature communications · 2017Article
- Conserved IKAROS-regulated genes associated with B-progenitor acute lymphoblastic leukemia outcome.The Journal of experimental medicine · 2017Article
- Conditional knockdown of BCL2A1 reveals rate-limiting roles in BCR-dependent B-cell survival.Cell death and differentiation · 2016Article
- An Inducible Retroviral Expression System for Tandem Affinity Purification Mass-Spectrometry-Based Proteomics Identifies Mixed Lineage Kinase Domain-like Protein (MLKL) as an Heat Shock Protein 90 (HSP90) Client.Molecular & cellular proteomics : MCP · 2016Article
- Stability of Doxycycline in Feed and Water and Minimal Effective Doses in Tetracycline-Inducible Systems.Journal of the American Association for Laboratory Animal Science : JAALAS · 2016Article
- Cohesin loss alters adult hematopoietic stem cell homeostasis, leading to myeloproliferative neoplasms.The Journal of experimental medicine · 2015Article
- Knockdown of PTHR1 in osteosarcoma cells decreases invasion and growth and increases tumor differentiation in vivo.Oncogene · 2015Article
- A transgenic mouse model to inducibly target prosurvival Bcl2 proteins with selective BH3 peptides in vivo.Cell death & disease · 2015Article
- Tyrosinase Depletion Prevents the Maturation of Melanosomes in the Mouse Hair Follicle.PloS one · 2015Article
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Authors and funding
9 authors.
Funding
Abstract
The tetracycline (tet)-regulated expression system allows for the inducible overexpression of protein-coding genes, or inducible gene knockdown based on expression of short hairpin RNAs (shRNAs). The system is widely used in mice, however it requires robust expression of a tet transactivator protein (tTA or rtTA) in the cell type of interest. Here we used an in vivo tet-regulated fluorescent reporter approach to characterise inducible gene/shRNA expression across a range of hematopoietic cell types of several commonly used transgenic tet transactivator mouse strains. We find that even in strains where the tet transactivator is expressed from a nominally ubiquitous promoter, the efficiency of tet-regulated expression can be highly variable between hematopoietic lineages and between differentiation stages within a lineage. In some cases tet-regulated reporter expression differs markedly between cells within a discrete, immunophenotypically defined population, suggesting mosaic transactivator expression. A recently developed CAG-rtTA3 transgenic mouse displays intense and efficient reporter expression in most blood cell types, establishing this strain as a highly effective tool for probing hematopoietic development and disease. These findings have important implications for interpreting tet-regulated hematopoietic phenotypes in mice, and identify mouse strains that provide optimal tet-regulated expression in particular hematopoietic progenitor cell types and mature blood lineages.
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