ArticlePloS one2013
Highly sensitive, highly specific whole-cell bioreporters for the detection of chromate in environmental samples.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 57 citations in OpenAlex.
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- Engineered probiotics that sequester arsenite in a mouse gastrointestinal system.bioRxiv : the preprint server for biology · 2026Article
- A review of biosensor for environmental monitoring: principle, application, and corresponding achievement of sustainable development goals.Bioengineered · 2023Review
- Electroactive Microorganisms in Advanced Energy Technologies.Molecules (Basel, Switzerland) · 2023Review
- Automated design of protein-binding riboswitches for sensing human biomarkers in a cell-free expression system.Nature communications · 2023Article
- Article
- Highly Sensitive Luminescent Bioassay Using RecombinantBiosensors · 2021Article
- In-situ monitoring of xenobiotics using genetically engineered whole-cell-based microbial biosensors: recent advances and outlook.World journal of microbiology & biotechnology · 2021Review
- Translating New Synthetic Biology Advances for Biosensing Into the Earth and Environmental Sciences.Frontiers in microbiology · 2020Review
- Two plant-hosted whole-cell bacterial biosensors for detection of bioavailable Cr(VI).World journal of microbiology & biotechnology · 2019Article
- Chromate tolerance and removal of bacterial strains isolated from uncontaminated and chromium-polluted environments.World journal of microbiology & biotechnology · 2019Article
- Impact of plant-associated bacteria biosensors on plant growth in the presence of hexavalent chromium.World journal of microbiology & biotechnology · 2017Article
- A Green Microbial Fuel Cell-Based Biosensor for In Situ Chromium (VI) Measurement in Electroplating Wastewater.Sensors (Basel, Switzerland) · 2017Article
- The Application of Whole Cell-Based Biosensors for Use in Environmental Analysis and in Medical Diagnostics.Sensors (Basel, Switzerland) · 2017Review
- Development and Application of a Synthetically-Derived Lead Biosensor Construct for Use in Gram-Negative Bacteria.Sensors (Basel, Switzerland) · 2016Article
- Utility of Ochrobactrum anthropi YC152 in a Microbial Fuel Cell as an Early Warning Device for Hexavalent Chromium Determination.Sensors (Basel, Switzerland) · 2016Article
- Use of Tunable Whole-Cell Bioreporters to Assess Bioavailable Cadmium and Remediation Performance in Soils.PloS one · 2016Article
- Escherichia [corrected] coli ribose binding protein based bioreporters revisited.Scientific reports · 2014Article
- Polyacrylamide hydrogel encapsulated E. coli expressing metal-sensing green fluorescent protein as a potential tool for copper ion determination.EXCLI journal · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial bioreporters offer excellent potentialities for the detection of the bioavailable portion of pollutants in contaminated environments, which currently cannot be easily measured. This paper describes the construction and evaluation of two microbial bioreporters designed to detect the bioavailable chromate in contaminated water samples. The developed bioreporters are based on the expression of gfp under the control of the chr promoter and the chrB regulator gene of TnOtChr determinant from Ochrobactrum tritici 5bvl1. pCHRGFP1 Escherichia coli reporter proved to be specific and sensitive, with minimum detectable concentration of 100 nM chromate and did not react with other heavy metals or chemical compounds analysed. In order to have a bioreporter able to be used under different environmental toxics, O. tritici type strain was also engineered to fluoresce in the presence of micromolar levels of chromate and showed to be as specific as the first reporter. Their applicability on environmental samples (spiked Portuguese river water) was also demonstrated using either freshly grown or cryo-preserved cells, a treatment which constitutes an operational advantage. These reporter strains can provide on-demand usability in the field and in a near future may become a powerful tool in identification of chromate-contaminated sites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.