ArticlePloS one2013
Functional innervation of Guinea-pig bladder interstitial cells of cajal subtypes: neurogenic stimulation evokes in situ calcium transients.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 27 citations in OpenAlex.
- Review
- Somatic mutations of CADM1 in aldosterone-producing adenomas and gap junction-dependent regulation of aldosterone production.Nature genetics · 2023Article
- Imatinib Mesylate Reduces Voiding Frequency in Female Mice With Acute Cyclophosphamide-Induced Cystitis.Frontiers in systems neuroscience · 2022Article
- Imatinib Mesylate Reduces Neurotrophic Factors and pERK and pAKT Expression in Urinary Bladder of Female Mice With Cyclophosphamide-Induced Cystitis.Frontiers in systems neuroscience · 2022Article
- Purinergic signalling in the urinary bladder - When function becomes dysfunction.Autonomic neuroscience : basic & clinical · 2021Article
- A Novel in situ Approach to Studying Detrusor Smooth Muscle Cells in Mice.Scientific reports · 2020Article
- Constitutively active HCN channels constrain detrusor excitability and modulate evoked contractions of human bladder.American journal of clinical and experimental urology · 2020Article
- What are the origins and relevance of spontaneous bladder contractions? ICI-RS 2017.Neurourology and urodynamics · 2018Review
- Modulation of Bladder Wall Micromotions Alters Intravesical Pressure Activity in the Isolated Bladder.Frontiers in physiology · 2018Article
- Acute radiation impacts contractility of guinea-pig bladder strips affecting mucosal-detrusor interactions.PloS one · 2018Article
- Article
- Low amplitude rhythmic contraction frequency in human detrusor strips correlates with phasic intravesical pressure waves.World journal of urology · 2017Article
- The Influence of Tetrodotoxin (TTX) on the Distribution and Chemical Coding of Caudal Mesenteric Ganglion (CaMG) Neurons Supplying the Porcine Urinary Bladder.Marine drugs · 2017Article
- Calcium Signaling in Interstitial Cells: Focus on Telocytes.International journal of molecular sciences · 2017Review
- Rhythmic Calcium Events in the Lamina Propria Network of the Urinary Bladder of Rat Pups.Frontiers in systems neuroscience · 2017Article
- Proliferation of Interstitial Cells in the Cyclophosphamide-Induced Cystitis and the Preventive Effect of Imatinib.BioMed research international · 2017Article
- Transient contractions of urinary bladder smooth muscle are drivers of afferent nerve activity during filling.The Journal of general physiology · 2016Article
- Neurogenic mechanisms in bladder and bowel ageing.Biogerontology · 2015Review
- Olfactory marker protein expression is an indicator of olfactory receptor-associated events in non-olfactory tissues.PloS one · 2015Article
- Calcium signalling in Cajal-like interstitial cells of the lower urinary tract.Nature reviews. Urology · 2014Review
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Authors and funding
4 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several populations of interstitial cells of Cajal (ICC) exist in the bladder, associated with intramural nerves. Although ICC respond to exogenous agonists, there is currently no evidence of their functional innervation. The objective was to determine whether bladder ICC are functionally innervated. Guinea-pig bladder tissues, loaded with fluo-4AM were imaged with fluorescent microscopy and challenged with neurogenic electrical field stimulation (EFS). All subtypes of ICC and smooth muscle cells (SMC) displayed spontaneous Ca(2+)-oscillations. EFS (0.5 Hz, 2 Hz, 10 Hz) evoked tetrodotoxin (1 µM)-sensitive Ca(2+)-transients in lamina propria ICC (ICC-LP), detrusor ICC and perivascular ICC (PICC) associated with mucosal microvessels. EFS responses in ICC-LP were significantly reduced by atropine or suramin. SMC and vascular SMC (VSM) also responded to EFS. Spontaneous Ca(2+)-oscillations in individual ICC-LP within networks occurred asynchronously whereas EFS evoked coordinated Ca(2+)-transients in all ICC-LP within a field of view. Non-correlated Ca(2+)-oscillations in detrusor ICC and adjacent SMC pre-EFS, contrasted with simultaneous neurogenic Ca(2+) transients evoked by EFS. Spontaneous Ca(2+)-oscillations in PICC were little affected by EFS, whereas large Ca(2+)-transients were evoked in pre-EFS quiescent PICC. EFS also increased the frequency of VSM Ca(2+)-oscillations. In conclusion, ICC-LP, detrusor ICC and PICC are functionally innervated. Interestingly, Ca(2+)-activity within ICC-LP networks and between detrusor ICC and their adjacent SMC were synchronous under neural control. VSM and PICC Ca(2+)-activity was regulated by bladder nerves. These novel findings demonstrate functional neural control of bladder ICC. Similar studies should now be carried out on neurogenic bladder to elucidate the contribution of impaired nerve-ICC communication to bladder pathophysiology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.