ArticlePloS one2013
Mapping the complex morphology of cell interactions with nanowire substrates using FIB-SEM.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 70 citations in OpenAlex.
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- Just a little prick: careful cell contacts enabled by ceramic nanostraws.Pflugers Archiv : European journal of physiology · 2026Article
- Cellular nanointerface of vertical nanostructure arrays and its applications.Nanoscale advances · 2022Review
- Advances in Cell-Conductive Polymer Biointerfaces and Role of the Plasma Membrane.Chemical reviews · 2022Article
- Tutorial: using nanoneedles for intracellular delivery.Nature protocols · 2021Review
- Robust neuronal differentiation of human iPSC-derived neural progenitor cells cultured on densely-spaced spiky silicon nanowire arrays.Scientific reports · 2021Article
- High Aspect Ratio and Light-Sensitive Micropillars Based on a Semiconducting Polymer Optically Regulate Neuronal Growth.ACS applied materials & interfaces · 2021Article
- Internalization and Viability Studies of Suspended Nanowire Silicon Chips in HeLa Cells.Nanomaterials (Basel, Switzerland) · 2020Article
- High-Aspect-Ratio Nanostructured Surfaces as Biological Metamaterials.Advanced materials (Deerfield Beach, Fla.) · 2020Review
- A nanostructure platform for live-cell manipulation of membrane curvature.Nature protocols · 2019Article
- Adhesion Stabilized en Masse Intracellular Electrical Recordings from Multicellular Assemblies.Nano letters · 2019Article
- Flexible elastomer patch with vertical silicon nanoneedles for intracellular and intratissue nanoinjection of biomolecules.Science advances · 2018Article
- Interfacing Cells with Vertical Nanoscale Devices: Applications and Characterization.Annual review of analytical chemistry (Palo Alto, Calif.) · 2018Review
- The Role of Membrane Curvature in Nanoscale Topography-Induced Intracellular Signaling.Accounts of chemical research · 2018Review
- Imaging analysis of the interface between osteoblasts and microrough surfaces of laser-sintered titanium alloy constructs.Journal of microscopy · 2018Article
- Revealing the Cell-Material Interface with Nanometer Resolution by Focused Ion Beam/Scanning Electron Microscopy.ACS nano · 2017Article
- Cellular uptake and dynamics of unlabeled freestanding silicon nanowires.Science advances · 2016Article
- Biodegradable nanoneedles for localized delivery of nanoparticles in vivo: exploring the biointerface.ACS nano · 2015Article
- Time-dependent subcellular distribution and effects of carbon nanotubes in lungs of mice.PloS one · 2015Article
- FIB-SEM imaging of carbon nanotubes in mouse lung tissue.Analytical and bioanalytical chemistry · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using high resolution focused ion beam scanning electron microscopy (FIB-SEM) we study the details of cell-nanostructure interactions using serial block face imaging. 3T3 Fibroblast cellular monolayers are cultured on flat glass as a control surface and on two types of nanostructured scaffold substrates made from silicon black (Nanograss) with low- and high nanowire density. After culturing for 72 hours the cells were fixed, heavy metal stained, embedded in resin, and processed with FIB-SEM block face imaging without removing the substrate. The sample preparation procedure, image acquisition and image post-processing were specifically optimised for cellular monolayers cultured on nanostructured substrates. Cells display a wide range of interactions with the nanostructures depending on the surface morphology, but also greatly varying from one cell to another on the same substrate, illustrating a wide phenotypic variability. Depending on the substrate and cell, we observe that cells could for instance: break the nanowires and engulf them, flatten the nanowires or simply reside on top of them. Given the complexity of interactions, we have categorised our observations and created an overview map. The results demonstrate that detailed nanoscale resolution images are required to begin understanding the wide variety of individual cells' interactions with a structured substrate. The map will provide a framework for light microscopy studies of such interactions indicating what modes of interactions must be considered.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.