ArticlePloS one2013
Comparison study of MS-HRM and pyrosequencing techniques for quantification of APC and CDKN2A gene methylation.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Trial
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- A case control study investigating the methylation levels of GHRL and GHSR genes in alcohol use disorder.Molecular biology reports · 2024Article
- Temperature-Wise Calibration Increases the Accuracy of DNA Methylation Levels Determined by High-Resolution Melting (HRM).International journal of molecular sciences · 2024Article
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- Maternal Epigenetic Dysregulation as a Possible Risk Factor for Neurodevelopmental Disorders.Genes · 2023Article
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- Mutational analysis of driver genes defines the colorectal adenoma: in situ carcinoma transition.Scientific reports · 2022Article
- Folic Acid Levels During Pregnancy Regulate Trophoblast Invasive Behavior and the Possible Development of Preeclampsia.Frontiers in nutrition · 2022Article
- Age estimation using methylation-sensitive high-resolution melting (MS-HRM) in both healthy felines and those with chronic kidney disease.Scientific reports · 2021Article
- Article
- Comparison of Bisulfite Pyrosequencing and Methylation-Specific qPCR for Methylation Assessment.International journal of molecular sciences · 2020Article
- DNA methylation analysis with methylation-sensitive high-resolution melting (MS-HRM) reveals gene panel for glioma characteristics.CNS neuroscience & therapeutics · 2020Article
- DNA hypomethylation of the Krüppel-like factor 11 (KLF11) gene promoter: a putative biomarker of depression comorbidity in panic disorder and of non-anxious depression?Journal of neural transmission (Vienna, Austria : 1996) · 2020Article
- Investigation ofFrontiers in molecular neuroscience · 2020Article
- Plasma Homocysteine and Polymorphisms of Genes Involved in Folate Metabolism Correlate withMetabolites · 2019Article
- DNA methylation detection methods used in colorectal cancer.World journal of clinical cases · 2019Review
- Association of Polymorphisms in Genes Involved in One-Carbon Metabolism withInternational journal of molecular sciences · 2019Article
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is increasing interest in the development of cost-effective techniques for the quantification of DNA methylation biomarkers. We analyzed 90 samples of surgically resected colorectal cancer tissues for APC and CDKN2A promoter methylation using methylation sensitive-high resolution melting (MS-HRM) and pyrosequencing. MS-HRM is a less expensive technique compared with pyrosequencing but is usually more limited because it gives a range of methylation estimates rather than a single value. Here, we developed a method for deriving single estimates, rather than a range, of methylation using MS-HRM and compared the values obtained in this way with those obtained using the gold standard quantitative method of pyrosequencing. We derived an interpolation curve using standards of known methylated/unmethylated ratio (0%, 12.5%, 25%, 50%, 75%, and 100% of methylation) to obtain the best estimate of the extent of methylation for each of our samples. We observed similar profiles of methylation and a high correlation coefficient between the two techniques. Overall, our new approach allows MS-HRM to be used as a quantitative assay which provides results which are comparable with those obtained by pyrosequencing.
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