ArticlePloS one2013
Primed pluripotent cell lines derived from various embryonic origins and somatic cells in pig.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 74 citations in OpenAlex.
- Defining core signaling pathways for supporting in vitro maintenance of pig extraembryonic endoderm (XEN) cells.Reproduction (Cambridge, England) · 2025Article
- Establishment of porcine embryonic stem cells in simplified serum free media and feeder free expansion.Stem cell research & therapy · 2024Article
- An optimized culture system for efficient derivation of porcine expanded potential stem cells from preimplantation embryos and by reprogramming somatic cells.Nature protocols · 2024Article
- The progress of induced pluripotent stem cells derived from pigs: a mini review of recent advances.Frontiers in cell and developmental biology · 2024Review
- NANOG expression in parthenogenetic porcine blastocysts is required for intact lineage specification and pluripotency.Animal bioscience · 2023Article
- Conserved features of non-primate bilaminar disc embryos and the germline.Stem cell reports · 2021Review
- Organoids in domestic animals: with which stem cells?Veterinary research · 2021Review
- IRF-1 expressed in the inner cell mass of the porcine early blastocyst enhances the pluripotency of induced pluripotent stem cells.Stem cell research & therapy · 2020Article
- Induced pluripotent stem cells from farm animals.Journal of animal science · 2020Review
- Establishment of porcine nuclear transfer-derived embryonic stem cells using induced pluripotent stem cells as donor nuclei.The Journal of reproduction and development · 2020Article
- Generation of transgene-free induced pluripotent stem cells from cardiac fibroblasts of goat embryos.Journal of stem cells & regenerative medicine · 2020Article
- Chemically Defined Media Can Maintain Pig Pluripotency Network In Vitro.Stem cell reports · 2019Article
- Establishment of porcine and human expanded potential stem cells.Nature cell biology · 2019Article
- Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis.Nature communications · 2019Article
- A six-inhibitor culture medium for improving naïve-type pluripotency of porcine pluripotent stem cells.Cell death discovery · 2019Article
- Identification and Characterization of theStem cells international · 2019Article
- Progress of stem/progenitor cell-based therapy for retinal degeneration.Journal of translational medicine · 2017Review
- Derivation of Porcine Embryonic Stem-Like Cells from In Vitro-Produced Blastocyst-Stage Embryos.Scientific reports · 2016Article
- Generation of liver-specific TGF-α/c-Myc-overexpressing porcine induced pluripotent stem-like cells and blastocyst formation using nuclear transfer.The Journal of veterinary medical science · 2016Article
- An Improved System for Generation of Diploid Cloned Porcine Embryos Using Induced Pluripotent Stem Cells Synchronized to Metaphase.PloS one · 2016Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since pluripotent embryonic stem cell (ESC) lines were first derived from the mouse, tremendous efforts have been made to establish ESC lines in several domestic species including the pig; however, authentic porcine ESCs have not yet been established. It has proven difficult to maintain an ESC-like state in pluripotent porcine cell lines due to the frequent occurrence of spontaneous differentiation into an epiblast stem cell (EpiSC)-like state during culture. We have been able to derive EpiSC-like porcine ESC (pESC) lines from blastocyst stage porcine embryos of various origins, including in vitro fertilized (IVF), in vivo derived, IVF aggregated, and parthenogenetic embryos. In addition, we have generated induced pluripotent stem cells (piPSCs) via plasmid transfection of reprogramming factors (Oct4, Sox2, Klf4, and c-Myc) into porcine fibroblast cells. In this study, we analyzed characteristics such as marker expression, pluripotency and the X chromosome inactivation status in female of our EpiSC-like pESC lines along with our piPSC line. Our results show that these cell lines demonstrate the expression of genes associated with the Activin/Nodal and FGF2 pathways along with the expression of pluripotent markers Oct4, Sox2, Nanog, SSEA4, TRA 1-60 and TRA 1-81. Furthermore all of these cell lines showed in vitro differentiation potential, the X chromosome inactivation in female and a normal karyotype. Here we suggest that the porcine species undergoes reprogramming into a primed state during the establishment of pluripotent stem cell lines.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.