ArticleNucleic acids research2013
Nap1 regulates proper CENP-B binding to nucleosomes.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Roles of histone chaperone Nap1 and histone acetylation in regulating phase-separation of nucleosome arrays.Nature communications · 2025Article
- Diverse mechanisms of centromere specification.Current biology : CB · 2021Review
- Encoded, click-reactive DNA-binding domains for programmable capture of specific chromatin segments.Chemical science · 2020Article
- A genetic memory initiates the epigenetic loop necessary to preserve centromere position.The EMBO journal · 2020Article
- H3K9me3 maintenance on a human artificial chromosome is required for segregation but not centromere epigenetic memory.Journal of cell science · 2020Article
- A Variant of the Histone-Binding Protein sNASP Contributes to Mouse Lupus.Frontiers in immunology · 2019Article
- The histone chaperone NAP1L3 is required for haematopoietic stem cell maintenance and differentiation.Scientific reports · 2018Article
- NAP1L1 accelerates activation and decreases pausing to enhance nucleosome remodeling by CSB.Nucleic acids research · 2017Article
- Rapid turnover of DnaA at replication origin regions contributes to initiation control of DNA replication.PLoS genetics · 2017Article
- Insight into G-quadruplex-hemin DNAzyme/RNAzyme: adjacent adenine as the intramolecular species for remarkable enhancement of enzymatic activity.Nucleic acids research · 2016Article
- Stable complex formation of CENP-B with the CENP-A nucleosome.Nucleic acids research · 2015Article
- Replication of alpha-satellite DNA arrays in endogenous human centromeric regions and in human artificial chromosome.Nucleic acids research · 2014Article
- Chromatin dynamics: H3K4 methylation and H3 variant replacement during development and in cancer.Cellular and molecular life sciences : CMLS · 2014Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
CENP-B is a widely conserved centromeric satellite DNA-binding protein, which specifically binds to a 17-bp DNA sequence known as the CENP-B box. CENP-B functions positively in the de novo assembly of centromeric nucleosomes, containing the centromere-specific histone H3 variant, CENP-A. At the same time, CENP-B also prevents undesired assembly of the CENP-A nucleosome through heterochromatin formation on satellite DNA integrated into ectopic sites. Therefore, improper CENP-B binding to chromosomes could be harmful. However, no CENP-B eviction mechanism has yet been reported. In the present study, we found that human Nap1, an acidic histone chaperone, inhibited the non-specific binding of CENP-B to nucleosomes and apparently stimulated CENP-B binding to its cognate CENP-B box DNA in nucleosomes. In human cells, the CENP-B eviction activity of Nap1 was confirmed in model experiments, in which the CENP-B binding to a human artificial chromosome or an ectopic chromosome locus bearing CENP-B boxes was significantly decreased when Nap1 was tethered near the CENP-B box sequence. In contrast, another acidic histone chaperone, sNASP, did not promote CENP-B eviction in vitro and in vivo and did not stimulate specific CENP-B binding to CENP-A nucleosomes in vitro. We therefore propose a novel mechanism of CENP-B regulation by Nap1.
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