ArticleNucleic acids research2013
Mutational analysis of the ribosome assembly GTPase RbgA provides insight into ribosome interaction and ribosome-stimulated GTPase activation.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Distinct steps of nuclear maturation of human pre-60S complexes require the activity of GTPases including GNL3L.Nucleic acids research · 2026Article
- The late stages of yeast mitoribosome large subunit biogenesis.Biochimica et biophysica acta. Molecular cell research · 2025Article
- A single 2'-O-methylation of ribosomal RNA gates assembly of a functional ribosome.Nature structural & molecular biology · 2023Article
- RbgA ensures the correct timing in the maturation of the 50S subunits functional sites.Nucleic acids research · 2022Article
- Role of GTPases in Driving Mitoribosome Assembly.Trends in cell biology · 2021Review
- Interconnected assembly factors regulate the biogenesis of mitoribosomal large subunit.The EMBO journal · 2021Article
- Structural consequences of the interaction of RbgA with a 50S ribosomal subunit assembly intermediate.Nucleic acids research · 2019Article
- The Impact of the Stringent Response on TRAFAC GTPases and Prokaryotic Ribosome Assembly.Cells · 2019Review
- A bacterial checkpoint protein for ribosome assembly moonlights as an essential metabolite-proofreading enzyme.Nature communications · 2019Article
- Structural basis for (p)ppGpp-mediated inhibition of the GTPase RbgA.The Journal of biological chemistry · 2018Article
- MTG1 couples mitoribosome large subunit assembly with intersubunit bridge formation.Nucleic acids research · 2018Article
- Functional characterization of chloroplast-targeted RbgA GTPase in higher plants.Plant molecular biology · 2017Article
- RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket.Nucleic acids research · 2017Article
- ppGpp negatively impacts ribosome assembly affecting growth and antimicrobial tolerance in Gram-positive bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Histidine 114 Is Critical for ATP Hydrolysis by the Universally Conserved ATPase YchF.The Journal of biological chemistry · 2015Article
- Mitochondrial ribosome assembly in health and disease.Cell cycle (Georgetown, Tex.) · 2015Review
- Functional interaction between ribosomal protein L6 and RbgA during ribosome assembly.PLoS genetics · 2014Article
- Predicting the minimal translation apparatus: lessons from the reductive evolution of mollicutes.PLoS genetics · 2014Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Ribosome biogenesis GTPase A protein (RbgA) is an essential GTPase required for the biogenesis of the 50S subunit in Bacillus subtilis. Homologs of RbgA are widely distributed in bacteria and eukaryotes and are implicated in ribosome assembly in the mitochondria, chloroplast and cytoplasm. Cells depleted of RbgA accumulate an immature large subunit that is missing key ribosomal proteins. RbgA, unlike many members of the Ras superfamily of GTPases, lacks a defined catalytic residue for carrying out guanosine triphosphate (GTP) hydrolysis. To probe RbgA function in ribosome assembly, we used a combined bioinformatics, genetic and biochemical approach. We identified a RNA-binding domain within the C-terminus of RbgA that is structurally similar to AmiR-NasR Transcription Anti-termination Regulator (ANTAR) domains, which are known to bind structured RNA. Mutation of key residues in the ANTAR domain altered RbgA association with the ribosome. We identified a putative catalytic residue within a highly conserved region of RbgA, His9, which is contained within a similar PGH motif found in elongation factor Tu (EF-Tu) that is required for GTP hydrolysis on interaction with the ribosome. Finally, our results support a model in which the GTPase activity of RbgA directly participates in the maturation of the large subunit rather than solely promoting dissociation of RbgA from the 50S subunit.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.