ArticleJournal of clinical microbiology2013
Quantitative real-time PCR assay panel for detection and type-specific identification of epidemic respiratory human adenoviruses.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The trial behind it
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Who cites it
32 citing papers in PubMed.
- Molecular Characterization of Human Adenoviruses Before and After a Severe HAdV-7 Military Outbreak in Finland in 2024.Influenza and other respiratory viruses · 2026Article
- Adenovirus Infections in Hematologic Malignancy and Hematopoietic Cell Transplant Recipients: Epidemiology, Diagnosis, and Management.Infection & chemotherapy · 2026Review
- Adenovirus Types in US Children Hospitalized or Seen in the Emergency Department With Acute Respiratory Illness, 2016-2019.Open forum infectious diseases · 2025Article
- Adaptation and validation of a gastrointestinal panel to detect diarrheal virus pathogens on a high-throughput qPCR system.Medical microbiology and immunology · 2025Article
- Rapid and sensitive detection of human adenovirus types 3 and 7 using CRISPR-Cas12b coupled with multiple cross displacement amplification.Infectious medicine · 2025Article
- Adenovirus type 7d outbreak associated with severe clinical presentation, Finland, February to June 2024.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2025Article
- A Novel Multiplex LAMP Assay for the Detection of Respiratory Human Adenoviruses.International journal of molecular sciences · 2024Article
- Human adenovirus outbreak at a university campus monitored by wastewater and clinical surveillance.medRxiv : the preprint server for health sciences · 2024Article
- Factors Associated with Severe Adenovirus Pneumonia in Children: A Retrospective Study from Guangzhou, China (2017-2019).Medical science monitor : international medical journal of experimental and clinical research · 2023Article
- Review
- [Silencing CD46 and DSG2 in host A549 cells inhibits entry of human adenovirus type 3 and type 7 and reduces interleukin-8 release].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2022Article
- Human Adenovirus Type 4 Comprises Two Major Phylogroups with Distinct Replicative Fitness and Virulence Phenotypes.Journal of virology · 2022Article
- Outbreaks of Adenovirus-associated Respiratory Illness on 5 College Campuses in the United States, 2018-2019.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Emerging antiviral therapeutics for human adenovirus infection: Recent developments and novel strategies.Antiviral research · 2021Review
- Human adenovirus species in children with acute respiratory illnesses.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2021Article
- Clinical Characteristics and Outcomes of Severe Pneumonia in Children Under 5 Years Old With and Without Adenovirus Infection in Guangzhou.Frontiers in pediatrics · 2021Article
- Outcomes of human adenovirus infection and disease in a retrospective cohort of pediatric solid organ transplant recipients.Pediatric transplantation · 2019Observational
- Perspective on Adenoviruses: Epidemiology, Pathogenicity, and Gene Therapy.Biomedicines · 2019Review
- Respiratory Illness Associated With Emergent Human Adenovirus Genome Type 7d, New Jersey, 2016-2017.Open forum infectious diseases · 2019Article
- Human adenovirus type 7 infection causes a more severe disease than type 3.BMC infectious diseases · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Outbreaks of human adenovirus (HAdV) acute respiratory illness (ARI) have been well documented among civilians and unvaccinated military recruits. Among the 7 recognized HAdV species (A to G), species B (particularly serotypes 3, 7, 11, 14, and 21) and E (serotype 4) have more often been associated with epidemic ARI. Rapid detection and type-specific identification of these viruses would enhance outbreak response and help guide prevention and control measures. To this end, we developed type-specific real-time quantitative PCR (qPCR) assays for HAdV types 3, 4, 7, 11, 14, 16, and 21 targeting the HAdV hexon gene. All type-specific qPCR assays reproducibly detected as few as 10 copies/reaction of quantified hexon recombinant plasmids with a linear dynamic range of 8 log units (10(1) to 10(8) copies); in contrast, a generic qPCR assay that detects all HAdV types run concurrently detected between 10 and 100 copies/reaction, depending on the virus type. No nonspecific amplifications were observed with concentrated nucleic acid from 51 HAdV prototype strains or other common respiratory pathogens. All members of a panel of 137 previously typed HAdV field isolates and positive clinical specimens were correctly characterized by the type-specific qPCR assays; two different HAdV types were detected in three of the clinical specimens and confirmed by amplicon sequencing. The qPCR assays permit sensitive, specific, and quantitative detection and identification of seven clinically important respiratory HAdVs and should provide a convenient adjunct to classical typing methods for a rapid response to HAdV outbreaks.
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