ArticleMolecular biology of the cell2013
Rab1b overexpression modifies Golgi size and gene expression in HeLa cells and modulates the thyrotrophin response in thyroid cells in culture.
Article in Molecular biology of the cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Candidate biomarkers in brown adipose tissue for post-mortem diagnosis of fatal hypothermia.International journal of legal medicine · 2024Article
- Rab1b-GBF1-ARF1 Secretory Pathway Axis Is Required for Birnavirus Replication.Journal of virology · 2022Article
- The Small GTPase FgRab1 Plays Indispensable Roles in the Vegetative Growth, Vesicle Fusion, Autophagy and Pathogenicity ofInternational journal of molecular sciences · 2022Article
- CREB3L2 Modulates Nerve Growth Factor-Induced Cell Differentiation.Frontiers in molecular neuroscience · 2021Article
- Rab proteins as major determinants of the Golgi complex structure.Small GTPases · 2018Review
- CREB3L1-mediated functional and structural adaptation of the secretory pathway in hormone-stimulated thyroid cells.Journal of cell science · 2017Article
- Rab1 interacts with GOLPH3 and controls Golgi structure and contractile ring constriction during cytokinesis in Drosophila melanogaster.Open biology · 2017Article
- Article
- Comparing the Primary and Recall Immune Response Induced by a New EV71 Vaccine Using Systems Biology Approaches.PloS one · 2015Article
- How Rab proteins determine Golgi structure.International review of cell and molecular biology · 2015Review
- Regulating the large Sec7 ARF guanine nucleotide exchange factors: the when, where and how of activation.Cellular and molecular life sciences : CMLS · 2014Review
- Reduction in Golgi apparatus dimension in the absence of a residential protein, N-acetylglucosaminyltransferase V.Histochemistry and cell biology · 2014Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
Rab1b belongs to the Rab-GTPase family that regulates membrane trafficking and signal transduction systems able to control diverse cellular activities, including gene expression. Rab1b is essential for endoplasmic reticulum-Golgi transport. Although it is ubiquitously expressed, its mRNA levels vary among different tissues. This work aims to characterize the role of the high Rab1b levels detected in some secretory tissues. We report that, in HeLa cells, an increase in Rab1b levels induces changes in Golgi size and gene expression. Significantly, analyses applied to selected genes, KDELR3, GM130 (involved in membrane transport), and the proto-oncogene JUN, indicate that the Rab1b increase acts as a molecular switch to control the expression of these genes at the transcriptional level, resulting in changes at the protein level. These Rab1b-dependent changes require the activity of p38 mitogen-activated protein kinase and the cAMP-responsive element-binding protein consensus binding site in those target promoter regions. Moreover, our results reveal that, in a secretory thyroid cell line (FRTL5), Rab1b expression increases in response to thyroid-stimulating hormone (TSH). Additionally, changes in Rab1b expression in FRTL5 cells modify the specific TSH response. Our results show, for the first time, that changes in Rab1b levels modulate gene transcription and strongly suggest that a Rab1b increase is required to elicit a secretory response.
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Registered trials
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