ArticleSensors (Basel, Switzerland)2013
G-quadruplex DNAzyme molecular beacon for amplified colorimetric biosensing of Pseudostellaria heterophylla.
Article in Sensors (Basel, Switzerland), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- A Novel Approach for Colorimetric Detection of Glyphosate in Food Based on a Split Aptamer Nanostructure and DNAzyme Activity.Journal of fluorescence · 2025Article
- A rapid and high sensitivity RNA detection based on NASBA and G4-ThT fluorescent biosensor.Scientific reports · 2022Article
- G-Quadruplexes as An Alternative Recognition Element in Disease-Related Target Sensing.Molecules (Basel, Switzerland) · 2019Review
- A split G-quadruplex and graphene oxide-based low-background platform for fluorescence authentication of Pseudostellaria heterophylla.Sensors (Basel, Switzerland) · 2014Article
- Nucleic acid detection using G-quadruplex amplification methodologies.Methods (San Diego, Calif.) · 2013Review
- G-quadruplexes as sensing probes.Molecules (Basel, Switzerland) · 2013Review
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Authors and funding
4 authors.
Funding
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Abstract
With an internal transcribed spacer of 18 S, 5.8 S and 26 S nuclear ribosomal DNA (nrDNA ITS) as DNA marker, we report a colorimetric approach for authentication of Pseudostellaria heterophylla (PH) and its counterfeit species based on the differentiation of the nrDNA ITS sequence. The assay possesses an unlabelled G-quadruplex DNAzyme molecular beacon (MB) probe, employing complementary sequence as biorecognition element and 1:1:1:1 split G-quadruplex halves as reporter. In the absence of target DNA (T-DNA), the probe can shape intermolecular G-quadruplex structures capable of binding hemin to form G-quadruplex-hemin DNAzyme and catalyze the oxidation of ABTS2- to blue-green ABTS•- by H(2)O(2). In the presence of T-DNA, T-DNA can hybridize with the complementary sequence to form a duplex structure, hindering the formation of the G-quadruplex structure and resulting in the loss of the catalytic activity. Consequently, a UV-Vis absorption signal decrease is observed in the ABTS2--H(2)O(2) system. The "turn-off" assay allows the detection of T-DNA from 1.0 × 10-9 to 3.0 × 10-7 mol·L-1 (R2 = 0.9906), with a low detection limit of 3.1 × 10-10 mol·L-1. The present study provides a sensitive and selective method and may serve as a foundation of utilizing the DNAzyme MB sensor for identifying traditional Chinese medicines.
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