ArticleBMC genomics2013
RNAi phenotypes are influenced by the genetic background of the injected strain.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Article
- The quest for the best target genes for RNAi-mediated pest control.Insect molecular biology · 2025Review
- Classification of and detection techniques for RNAi-induced effects in GM plants.Frontiers in plant science · 2025Review
- Phenotypic screen and transcriptomics approach complement each other in functional genomics of defensive stink gland physiology.BMC genomics · 2022Article
- Screens in fly and beetle reveal vastly divergent gene sets required for developmental processes.BMC biology · 2022Article
- Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide.Frontiers in zoology · 2021Review
- Article
- Enhancing RNAi Efficiency to Decipher the Functional Response of Potential Genes inFrontiers in physiology · 2020Article
- Assessing the Risks of Topically Applied dsRNA-Based Products to Non-target Arthropods.Frontiers in plant science · 2020Article
- Unpredictable Effects of the Genetic Background of Transgenic Lines in Physiological Quantitative Traits.G3 (Bethesda, Md.) · 2019Article
- A Role forDevelopment & reproduction · 2019Article
- A morphological novelty evolved by co-option of a reduced gene regulatory network and gene recruitment in a beetle.Proceedings. Biological sciences · 2018Article
- UTR-specific knockdown of Distal-less and Sp8 leads to new phenotypic variants in the flour beetle Tribolium.Development genes and evolution · 2018Article
- A key role forDevelopment (Cambridge, England) · 2017Article
- RNAi technology: a new platform for crop pest control.Physiology and molecular biology of plants : an international journal of functional plant biology · 2017Review
- Article
- Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus.Journal of visualized experiments : JoVE · 2016Article
- Knockout of the Ribonuclease Inhibitor Gene Leaves Human Cells Vulnerable to Secretory Ribonucleases.Biochemistry · 2016Article
- Gene Disruption Technologies Have the Potential to Transform Stored Product Insect Pest Control.Insects · 2016Review
- The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology.Nature communications · 2015Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundRNA interference (RNAi) is a powerful tool to study gene function in organisms that are not amenable to classical forward genetics. Hence, together with the ease of comprehensively identifying genes by new generation sequencing, RNAi is expanding the scope of animal species and questions that can be addressed in terms of gene function. In the case of genetic mutants, the genetic background of the strains used is known to influence the phenotype while this has not been described for RNAi experiments.
resultsHere we show in the red flour beetle Tribolium castaneum that RNAi against Tc-importin α1 leads to different phenotypes depending on the injected strain. We rule out off target effects and show that sequence divergence does not account for this difference. By quantitatively comparing phenotypes elicited by RNAi knockdown of four different genes we show that there is no general difference in RNAi sensitivity between these strains. Finally, we show that in case of Tc-importin α1 the difference depends on the maternal genotype.
conclusionsThese results show that in RNAi experiments strain specific differences have to be considered and that a proper documentation of the injected strain is required. This is especially important for the increasing number of emerging model organisms that are being functionally investigated using RNAi. In addition, our work shows that RNAi is suitable to systematically identify the differences in the gene regulatory networks present in populations of the same species, which will allow novel insights into the evolution of animal diversity.
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