ArticleRadiation and environmental biophysics2013
Are mouse lens epithelial cells more sensitive to γ-irradiation than lymphocytes?
Article in Radiation and environmental biophysics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 27 citations in OpenAlex.
- Slightly increased level of DNA migration in the comet assay: does statistical significance equal biological significance?Mutagenesis · 2025Review
- Methodologies to unlock the molecular expression and cellular structure of ocular lens epithelial cells.Frontiers in cell and developmental biology · 2022Article
- Heterozygous Loss of Yap1 in Mice Causes Progressive Cataracts.Investigative ophthalmology & visual science · 2020Article
- Inverse dose-rate effect of ionising radiation on residual 53BP1 foci in the eye lens.Scientific reports · 2019Article
- Lifetime study in mice after acute low-dose ionizing radiation: a multifactorial study with special focus on cataract risk.Radiation and environmental biophysics · 2018Article
- Article
- Nonlinear ionizing radiation-induced changes in eye lens cell proliferation, cyclin D1 expression and lens shape.Open biology · 2015Article
- Radiation-induced alterations of histone post-translational modification levels in lymphoblastoid cell lines.Radiation oncology (London, England) · 2014Article
- Epithelial cells as alternative human biomatrices for comet assay.Frontiers in genetics · 2014Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this pilot study we compared for the first time the radiation sensitivity of mouse lens epithelial cells (LECs) and mouse lymphocytes. We freshly prepared LECs and lymphocytes and irradiated them with γ-rays ((137)Cs; doses ranging from 0.25 to 2 Gy). DNA damage and repair were evaluated by alkaline comet assay and γH2AX foci assay. Using the comet assay, we observed a dose-dependent increase in DNA damage in both cell types. The faster formation of single- and double-strand breaks in LECs of C57BL/6 mice at doses below 1 Gy needs to be confirmed in other mouse strains. Immunofluorescence for γH2AX foci showed a higher degree of lesions in LECs from C57BL/6J mice compared to those of JF1 mice and to lymphocytes of both strains. Correspondingly, repair of DNA damage proceeded faster in LECs of C57BL/6J mice compared to LECs of JF1 mice and lymphocytes of both strains. It is obvious that the lymphocytes of both strains repaired DNA lesions more slowly than the corresponding LECs. In conclusion, our results demonstrate that LECs of C57Bl/6 mice show a steeper dose-response than lymphocytes in both types of experiments. It shows that both test systems are able to be used also at doses below 0.25 Gy. The observed difference in DNA repair between the LECs from C57BL/6J mice compared to the LECs from JF1 mice and to the lymphocytes of both strains warrants further experiments to identify the underlying molecular mechanisms.
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