ArticlePlant physiology2013
Structure-guided engineering of plant phytochrome B with altered photochemistry and light signaling.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 55 citations in OpenAlex.
- Interactive Effects of Genes and Environment on Vegetative and Reproductive Tradeoffs of Leaf Size Mutants.Plant-environment interactions (Hoboken, N.J.) · 2026Article
- Hypsochromic shift in phytochrome C Pr absorption complements phytochrome B-mediated inhibition of hypocotyl elongation.The Plant journal : for cell and molecular biology · 2026Article
- Intragenic suppressor screen of YHB identifies novel and known loss-of-function alleles of Arabidopsis phytochrome B.bioRxiv : the preprint server for biology · 2026Article
- Production of Plant Phytochromes by Combining Insect Cells and Bacterial Expression Systems.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Overcoming the Challenges of Producing Plant Phytochromes in Escherichia coli.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Contrasting interactions between photon spectra and temperature in cold-sensitive basil and cold-tolerant lettuce.Frontiers in plant science · 2025Article
- Exploiting fourth-generation synchrotron radiation for enzyme and photoreceptor characterization.IUCrJ · 2025Article
- Photobody formation spatially segregates two opposing phytochrome B signaling actions of PIF5 degradation and stabilization.Nature communications · 2024Article
- Phytochrome B enhances seed germination tolerance to high temperature by reducing S-nitrosylation of HFR1.EMBO reports · 2022Article
- Differing biophysical properties underpin the unique signaling potentials within the plant phytochrome photoreceptor families.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Beyond natural: synthetic expansions of botanical form and function.The New phytologist · 2020Review
- Spectral and photochemical diversity of tandem cysteine cyanobacterial phytochromes.The Journal of biological chemistry · 2020Article
- Regulation of monocot and dicot plant development with constitutively active alleles of phytochrome B.Plant direct · 2020Article
- Molecular mechanisms underlying phytochrome-controlled morphogenesis in plants.Nature communications · 2019Review
- Characterization of Maize Phytochrome-Interacting Factors in Light Signaling and Photomorphogenesis.Plant physiology · 2019Article
- On the (un)coupling of the chromophore, tongue interactions, and overall conformation in a bacterial phytochrome.The Journal of biological chemistry · 2018Article
- PCH1 and PCHL promote photomorphogenesis in plants by controlling phytochrome B dark reversion.Nature communications · 2017Article
- Photosensing and Thermosensing by Phytochrome B Require Both Proximal and Distal Allosteric Features within the Dimeric Photoreceptor.Scientific reports · 2017Article
- Light-induced Changes in the Dimerization Interface of Bacteriophytochromes.The Journal of biological chemistry · 2015Article
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Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytochromes (phys) encompass a diverse collection of biliproteins that enable cellular light perception by photoconverting between a red-light-absorbing ground state (Pr) and a far-red light-absorbing active state (Pfr). Based on the central role of plant phys in controlling numerous agriculturally important processes, their rational redesign offers great promise toward accelerating crop improvement. Employing as templates the available three-dimensional models of the photosensory module within bacterial phys, we report here our initial attempt to apply structure-guided mutagenesis to phy engineering using Arabidopsis (Arabidopsis thaliana) phyB, the dominant isoform in light-grown plants, as the example. A collection of phyB mutants was generated affecting the bilin-binding pocket that altered photochemistry, thermal stability, and/or nuclear localization patterns, some of which also impacted phenotypic outputs. Of particular interest are the Y361F substitution, which created Arabidopsis plants with greatly enhanced light sensitivity, mutants variably altered in Pfr-to-Pr thermal reversion and nuclear aggregation, and the D307A substitution, which failed to photoconvert from Pr to Pfr and display light-induced nuclear aggregation but retained some biological activity and accelerated turnover in red light. Taken together, this collection provides variants potentially useful to agriculture as well as new tools to better understand the molecular mechanisms underpinning phy signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.