ArticleMolecular cytogenetics2013
Trisomy 8: a common finding in mouse embryonic stem (ES) cell lines.
Article in Molecular cytogenetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- Article
- Chromosome X dosage modulates development of aneuploidy in genetically diverse mouse embryonic stem cells.Stem cell reports · 2026Article
- Embryo quality control via lineage-specific aneuploid cell elimination in embryos and stem cell-derived embryo models.Nature communications · 2026Article
- Cdk8 and Hira mutations trigger X chromosome elimination in naive female hybrid mouse embryonic stem cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2024Article
- Transformation of primary murine peritoneal mast cells by constitutive KIT activation is accompanied by loss ofFrontiers in immunology · 2023Article
- The RS4;11 cell line as a model for leukaemia with t(4;11)(q21;q23): Revised characterisation of cytogenetic features.Cancer reports (Hoboken, N.J.) · 2019Article
- Derivation and maintenance of mouse haploid embryonic stem cells.Nature protocols · 2019Article
- Aneuploidy screening of embryonic stem cell clones by metaphase karyotyping and droplet digital polymerase chain reaction.BMC cell biology · 2016Article
- Achilles' heel of pluripotent stem cells: genetic, genomic and epigenetic variations during prolonged culture.Cellular and molecular life sciences : CMLS · 2016Review
- Failure to replicate the STAP cell phenomenon.Nature · 2015Article
- Beyond the mouse monopoly: studying the male germ line in domestic animal models.ILAR journal · 2015Review
- Quality control method for RNA-seq using single nucleotide polymorphism allele frequency.Genes to cells : devoted to molecular & cellular mechanisms · 2014Article
- Chromosome instability in mouse embryonic stem cells.Scientific reports · 2014Article
- Fast quantitative real-time PCR-based screening for common chromosomal aneuploidies in mouse embryonic stem cells.Stem cell reports · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelled
backgroundObtaining a germ cell line is one of the most important steps in developing a transgenic or knockout mouse with a targeted mutated gene of interest. A common problem with this technology is that embryonic stem (ES) cells often lack, or are extremely inefficient at, germ line transmission.
resultsTo determine whether chromosomal anomalies are correlated with inefficient ES cell germ line transmission, we examined 97 constructed ES cell lines using conventional cytogenetic analysis, and fluorescence in situ hybridization (FISH). Chromosomal abnormalities occurred in 44 (45%) out of the 97 specimens analyzed: 31 specimens had trisomy 8 or mosaic trisomy 8, eight specimens had partial trisomy 8 resulting from unbalanced translocations, and five specimens had other chromosomal anomalies.
conclusionsOur data suggest that chromosomal analysis is an important tool for improving the yield and quality of gene targeting experiments.
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Registered trials
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