ArticleJournal of tissue engineering and regenerative medicine2015
eNOS transfection of adipose-derived stem cells yields bioactive nitric oxide production and improved results in vascular tissue engineering.
Article in Journal of tissue engineering and regenerative medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Tissue-Engineered Grafts from Human Decellularized Extracellular Matrices: A Systematic Review and Future Perspectives.International journal of molecular sciences · 2018Pooled it
- Nanotechnology Innovations in Pediatric Cardiology and Cardiovascular Medicine: A Comprehensive Review.Biomedicines · 2024Review
- Amelioration of aflatoxin acute hepatitis rat model by bone marrow mesenchymal stem cells and their hepatogenic differentiation.Veterinary world · 2022Article
- Cellularized small-caliber tissue-engineered vascular grafts: looking for the ultimate gold standard.NPJ Regenerative medicine · 2021Review
- Systematic Review of Tissue-Engineered Vascular Grafts.Frontiers in bioengineering and biotechnology · 2021Review
- Vascular Tissue Engineering: Challenges and Requirements for an Ideal Large Scale Blood Vessel.Frontiers in bioengineering and biotechnology · 2021Review
- Nitric Oxide-Producing Cardiovascular Stent Coatings for Prevention of Thrombosis and Restenosis.Frontiers in bioengineering and biotechnology · 2020Review
- Current understanding of adipose-derived mesenchymal stem cell-based therapies in liver diseases.Stem cell research & therapy · 2019Review
- Review of risk factors, treatment, and prevention of saphenous vein graft disease after coronary artery bypass grafting.The Journal of international medical research · 2018Review
- In Vivo Performance of Decellularized Vascular Grafts: A Review Article.International journal of molecular sciences · 2018Review
- Vascular Tissue Engineering: Progress, Challenges, and Clinical Promise.Cell stem cell · 2018Review
- Histone deacetylase inhibitors promote eNOS expression in vascular smooth muscle cells and suppress hypoxia-induced cell growth.Journal of cellular and molecular medicine · 2017Article
- Delivery of Antioxidant and Anti-inflammatory Agents for Tissue Engineered Vascular Grafts.Frontiers in pharmacology · 2017Review
- Article
- Proangiogenic Features of Mesenchymal Stem Cells and Their Therapeutic Applications.Stem cells international · 2016Review
- Sphingosine-1-phosphate promotes the differentiation of adipose-derived stem cells into endothelial nitric oxide synthase (eNOS) expressing endothelial-like cells.Journal of biomedical science · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
This study evaluates the durability of a novel tissue engineered blood vessel (TEBV) created by seeding a natural vascular tissue scaffold (decellularized human saphenous vein allograft) with autologous adipose-derived stem cells (ASC) differentiated into endothelial-like cells. Previous work with this model revealed the graft to be thrombogenic, likely due to inadequate endothelial differentiation as evidenced by minimal production of nitric oxide (NO). To evaluate the importance of NO expression by the seeded cells, we created TEBV using autologous ASC transfected with the endothelial nitric oxide synthase (eNOS) gene to produce NO. We found that transfected ASC produced NO at levels similar to endothelial cell (EC) controls in vitro which was capable of causing vasorelaxation of aortic specimens ex vivo. TEBV (n = 5) created with NO-producing ASC and implanted as interposition grafts within the aorta of rabbits remained patent for two months and demonstrated a non-thrombogenic surface compared to unseeded controls (n = 5). Despite the xenograft nature of the scaffold, the TEBV structure remained well preserved in seeded grafts. In sum, this study demonstrates that upregulation of NO expression within adult stem cells differentiated towards an endothelial-like lineage imparts a non-thrombogenic phenotype and highlights the importance of NO production by cells to be used as endothelial cell substitutes in vascular tissue engineering applications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.