ArticleCell biology international2013
Isolation and in vitro propagation of human skeletal muscle progenitor cells from fetal muscle.
Article in Cell biology international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- Sphere-Based Expansion of Myogenic Progenitors from Human Pluripotent Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Expandable Lung Epithelium Differentiated from Human Embryonic Stem Cells.Tissue engineering and regenerative medicine · 2022Article
- Article
- Cell Surface Proteins for Enrichment andStem cells international · 2022Article
- Microcarriers for Upscaling Cultured Meat Production.Frontiers in nutrition · 2020Review
- A cGMP-applicable expansion method for aggregates of human neural stem and progenitor cells derived from pluripotent stem cells or fetal brain tissue.Journal of visualized experiments : JoVE · 2014Article
- Derivation of myogenic progenitors directly from human pluripotent stem cells using a sphere-based culture.Stem cells translational medicine · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Skeletal muscle progenitor cells (SMPCs) are considered one of the most valuable cells for cell-based therapy targeting skeletal muscle. However, an efficient protocol for isolating and maintaining human myogenic progenitors in vitro has not been fully established. In this study, we demonstrate that human myogenic progenitors can be expanded and proliferated from human fetal muscles. Human SMPCs were prepared from fetal hind limb muscles and induced to proliferate as free-floating spheres termed myospheres in the medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Both myogenic progenitors and myoblast populations from human fetal muscles were effectively propagated in myospheres and passaged by a mechanical chopping. After expanding these spheres in culture, we tested whether myogenic progenitor cells can differentiate into multinucleated myotubes. The myospheres were dissociated, plated down on coverslips and cultured in the medium for terminal differentiation. We could confirm that the plated cells formed well-developed, multinucleated myotubes. This culture method using myospheres is an effective protocol to isolate and maintain SMPCs from human fetal skeletal muscles in culture.
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Registered trials
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