Evidence map›Paper›PMID 23319266›Full record

ArticleParasitology research2013

Acanthamoeba castellanii cysts: new ultrastructural findings.

Bibiana Chávez-Munguía, Lizbeth Salazar-Villatoro, Anel Lagunes-Guillén, Maritza Omaña-Molina, Martha Espinosa-Cantellano, Adolfo Martínez-Palomo

Abstract read
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In one paragraph

Article in Parasitology research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Observational
  3. Encystment and Excystment Processes inPathogens (Basel, Switzerland) · 2025
    Review
  4. Review
  5. Parasitology · 2020
    Article
  6. Frontiers in cellular and infection microbiology · 2020
    Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Bibiana Chávez-MunguíaDepartment of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, Av. IPN 2508, Zacatenco, 07360, Mexico City, Mexico. bchavez@cinvestav.mx
Lizbeth Salazar-Villatoro
Anel Lagunes-Guillén
Maritza Omaña-Molina
Martha Espinosa-Cantellano
Adolfo Martínez-Palomo
Instituto Politécnico Nacional · MX

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

During Acanthamoeba castellanii trophozoite-cysts differentiation, four morphological stages were identified by scanning electron microscopy: trophozoite, precyst, immature cysts, and mature cysts. Fluorescence microscopy reveals the presence of small cumulus of actin in the cytoplasm of precysts after treatment with rhodamine phalloidin. By the contrary, in mature cysts, fluorescence was not observed. However, when excystation was induced, large fluorescent patches were present. By transmission electron microscopy, encysting amebas showed small cytoplasmic vesicles containing fibrillar material, surrounded by a narrow area of thin fibrils. Similar appearance was observed in pseudopods and phagocytic invaginations. In addition, large aggregates of rod-shape elements, similar to the chromatoid bodies, described in other amebas, were present in the cytoplasm. These cysts presented large areas with orange fluorescence after treatment with acridine orange.

Indexed as

Acanthamoeba castellaniiMicroscopy, Electron, ScanningMicroscopy, Electron, TransmissionMicroscopy, FluorescenceSpores, Protozoan

Identifiers

PMID23319266
OpenAlexW2145550495

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.