ArticleAnnals of the Royal College of Surgeons of England2013
Nucleotide metabolic mismatches in mammalian hearts: implications for transplantation.
Article in Annals of the Royal College of Surgeons of England, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 2 citations in OpenAlex.
- Physiological basis for xenotransplantation from genetically modified pigs to humans.Physiological reviews · 2024Review
- Nucleotide Catabolism on the Surface of Aortic Valve Xenografts; Effects of Different Decellularization Strategies.Journal of cardiovascular translational research · 2016Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionHuman donor organ shortages have led surgeons and scientists to explore the use of animals as alternative organ sources. Acute thrombovascular rejection (AVR) is the main hurdle in xenotransplantation. disparities in nucleotide metabolism in the vessels of different species may contribute significantly to the microvascular component of AVR.
methodsWe evaluated the extent of nucleotide metabolism mismatch in selected organs and endothelial cells of different mammals with particular focus on the changes in activity of ecto-5'-nucleotidase (E5'n) elicited by exposure of porcine hearts or endothelial cells to human blood (ex vivo) or human plasma (in vitro).
resultsE5'n activity in the rat heart was significantly higher than in other species. We noted a significant difference (p<0.001) in E5'n activity between human and pig endothelial cell lines. Initial pig aortic endothelial E5'n activity decreased in vitro after a three-hour exposure to human and porcine plasma while remaining constant in controls. ex vivo perfusion with fresh human blood for four hours resulted in a significant decrease of E5'n activity in both wild type and transgenic pig hearts overexpressing human decay accelerating factor (p<0.001).
conclusionsThis study provides evidence that mismatches in basal mammalian metabolic pathways and humoral immunity interact in a xenogeneic environment. understanding the role of nucleotide metabolism and signalling in xenotransplantation may identify new targets for genetic modifications and may lead to the development of new therapies extending graft survival.
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