ArticleStem cells and development2013
Stress induces AMPK-dependent loss of potency factors Id2 and Cdx2 in early embryos and stem cells [corrected].
Article in Stem cells and development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- The integrated developmental stress response (ISR-DASR) shift reveals a functional transition from cellular to stem cell organismal adaptation.Scientific reports · 2026Article
- Novel kinetic and developmental transcriptomic pan-stress responses by embryonic stem cells.Scientific reports · 2025Article
- Mitochondrial Calcium uniporters are essential for meiotic progression in mouse oocytes by controlling CaCell proliferation · 2021Article
- Article
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- Stress Forces First Lineage Differentiation of Mouse Embryonic Stem Cells; Validation of a High-Throughput Screen for Toxicant Stress.Stem cells and development · 2019Article
- Prenatal transportation stress alters genome-wide DNA methylation in suckling Brahman bull calves.Journal of animal science · 2018Article
- Follistatin supplementation during in vitro embryo culture improves developmental competence of bovine embryos produced using sex-sorted semen.Reproductive biology · 2018Article
- Why AMPK agonists not known to be stressors may surprisingly contribute to miscarriage or hinder IVF/ART.Journal of assisted reproduction and genetics · 2018Review
- Two-cell embryos are more sensitive than blastocysts to AMPK-dependent suppression of anabolism and stemness by commonly used fertility drugs, a diet supplement, and stress.Journal of assisted reproduction and genetics · 2017Article
- CoQ10 increases mitochondrial mass and polarization, ATP and Oct4 potency levels, and bovine oocyte MII during IVM while decreasing AMPK activity and oocyte death.Journal of assisted reproduction and genetics · 2017Article
- Treatment with AICAR inhibits blastocyst development, trophectoderm differentiation and tight junction formation and function in mice.Molecular human reproduction · 2017Article
- Using stem cell oxygen physiology to optimize blastocyst culture while minimizing hypoxic stress.Journal of assisted reproduction and genetics · 2017Article
- Blastocyst-Derived Stem Cell Populations under Stress: Impact of Nutrition and Metabolism on Stem Cell Potency Loss and Miscarriage.Stem cell reviews and reports · 2017Review
- AMPK Knockdown in Placental Labyrinthine Progenitor Cells Results in Restriction of Critical Energy Resources and Terminal Differentiation Failure.Stem cells and development · 2017Article
- AMPK improves gut epithelial differentiation and barrier function via regulating Cdx2 expression.Cell death and differentiation · 2017Article
- Departure from optimal OThe Journal of reproduction and development · 2017Article
- Commonly used fertility drugs, a diet supplement, and stress force AMPK-dependent block of stemness and development in cultured mammalian embryos.Journal of assisted reproduction and genetics · 2016Article
- Development and Validation of a Rex1-RFP Potency Activity Reporter Assay That Quantifies Stress-Forced Potency Loss in Mouse Embryonic Stem Cells.Stem cells and development · 2016Article
- Endoplasmic reticulum stress signaling in mammalian oocytes and embryos: life in balance.International review of cell and molecular biology · 2015Review
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The AMP-activated protein kinase (AMPK) mediates rapid, stress-induced loss of the inhibitor of differentiation (Id)2 in blastocysts and trophoblast stem cells (TSC), and a lasting differentiation in TSC. However, it is not known if AMPK regulates other potency factors or regulates them before the blastocyst stage. The caudal-related homeodomain protein (Cdx)2 is a regulatory gene for determining TSC, the earliest placental lineage in the preimplantation mouse embryo, but is expressed in the oocyte and in early cleavage stage embryos before TSC arise. We assayed the expression of putative potency-maintaining phosphorylated Cdx2 ser60 in the oocyte, two-cell stage embryo, blastocyst, and in TSC. We studied the loss of Cdx2 phospho ser60 expression induced by hyperosmolar stress and its underlying mechanisms. Hyperosmolar stress caused rapid loss of nuclear Cdx2 phospho ser60 and Id2 in the two-cell stage embryo by 0.5 h. Stress-induced Cdx2 phospho ser60 and Id2 loss is reversed by the AMPK inhibitor compound C and is induced by the AMPK agonist 5-amino-1-β-d-ribofuranosyl-imidazole-4-carboxamide in the absence of stress. In the two-cell stage embryo and TSC hyperosmolar, stress caused AMPK-mediated loss of Cdx2 phospho ser60 as detected by immunofluorescence and immunoblot. We propose that AMPK may be the master regulatory enzyme for mediating stress-induced loss of potency as AMPK is also required for stress-induced loss of Id2 in blastocysts and TSC. Since AMPK mediates potency loss in embryos and stem cells it will be important to measure, test mechanisms for, and manage the AMPK function to optimize the stem cell and embryo quality in vitro and in vivo.
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