ArticleComparative and functional genomics2012
Gemi: PCR primers prediction from multiple alignments.
Article in Comparative and functional genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed.
- Genome sequences of human coronavirus NL63 diagnosed in southern France.Microbiology resource announcements · 2026Article
- Five-Year (2017-2022) Evolutionary Dynamics of Human Coronavirus HKU1 in Southern France With Emergence of Viruses Harboring Spike H512R Substitution.Journal of medical virology · 2025Article
- FBPP: software to design PCR primers and probes for nucleic acid base detection of foodborne pathogens.Scientific reports · 2024Article
- rprimer: an R/bioconductor package for design of degenerate oligos for sequence variable viruses.BMC bioinformatics · 2022Article
- Article
- Frankia-Enriched Metagenomes from the Earliest Diverging Symbiotic Frankia Cluster: They Come in Teams.Genome biology and evolution · 2019Article
- Article
- Article
- Shetti, a simple tool to parse, manipulate and search large datasets of sequences.Microbial genomics · 2015Article
- Identification of giant Mimivirus protein functions using RNA interference.Frontiers in microbiology · 2015Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Designing primers and probes for polymerase chain reaction (PCR) is a preliminary and critical step that requires the identification of highly conserved regions in a given set of sequences. This task can be challenging if the targeted sequences display a high level of diversity, as frequently encountered in microbiologic studies. We developed Gemi, an automated, fast, and easy-to-use bioinformatics tool with a user-friendly interface to design primers and probes based on multiple aligned sequences. This tool can be used for the purpose of real-time and conventional PCR and can deal efficiently with large sets of sequences of a large size.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.