ArticleJournal of bacteriology2013
Product-mediated regulation of pentalenolactone biosynthesis in Streptomyces species by the MarR/SlyA family activators PenR and PntR.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Regulatory Roles of a Novel MarR-like Protein Lmo0840 in Tetracycline-Associated Growth Phenotype and Virulence ofMicroorganisms · 2026Article
- Impact of the Transcriptional Regulator SCO7424 Overexpression on Antibiotic Production inAntibiotics (Basel, Switzerland) · 2026Article
- Redox control of antibiotic biosynthesis.mBio · 2025Article
- Cytochrome P450-catalyzed allylic oxidation of pentalenene to 1-deoxypentalenic acid in pentalenolactone biosynthesis.Engineering microbiology · 2025Article
- Sesquiterpenes fromMarine drugs · 2023Article
- Actinomycetes as Producers of Biologically Active Terpenoids: Current Trends and Patents.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Diverse MarR bacterial regulators of auxin catabolism in the plant microbiome.Nature microbiology · 2022Article
- A Regulator Based "Semi-Targeted" Approach to Activate Silent Biosynthetic Gene Clusters.International journal of molecular sciences · 2021Article
- Joint engineering of SACE_Lrp and its target MarR enhances the biosynthesis and export of erythromycin in Saccharopolyspora erythraea.Applied microbiology and biotechnology · 2021Article
- Synthetic biology, combinatorial biosynthesis, and chemo‑enzymatic synthesis of isoprenoids.Journal of industrial microbiology & biotechnology · 2020Review
- CtcS, a MarR family regulator, regulates chlortetracycline biosynthesis.BMC microbiology · 2019Article
- Regulation of antibiotic biosynthesis in actinomycetes: Perspectives and challenges.Synthetic and systems biotechnology · 2018Review
- SAV4189, a MarR-Family Regulator inFrontiers in microbiology · 2018Article
- Mechanistic studies of DepR in regulating FK228 biosynthesis in Chromobacterium violaceum no. 968.PloS one · 2018Article
- Anti-Inflammatory Dembranoids from the Soft Coral Lobophytum crassum.Marine drugs · 2017Article
- Structural and Chemical Biology of Terpenoid Cyclases.Chemical reviews · 2017Review
- Regulation of Metabolic Pathways by MarR Family Transcription Factors.Computational and structural biotechnology journal · 2017Review
- Substitution of a Single Amino Acid Reverses the Regiospecificity of the Baeyer-Villiger Monooxygenase PntE in the Biosynthesis of the Antibiotic Pentalenolactone.Biochemistry · 2016Article
- Identification and activation of novel biosynthetic gene clusters by genome mining in the kirromycin producer Streptomyces collinus Tü 365.Journal of industrial microbiology & biotechnology · 2016Article
- A MarR Family Transcriptional Regulator, DptR3, Activates Daptomycin Biosynthesis and Morphological Differentiation in Streptomyces roseosporus.Applied and environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
The orthologous penR and pntR genes from the pentalenolactone biosynthetic gene clusters of Streptomyces exfoliatus UC5319 and S. arenae TÜ469, respectively, were predicted to encode MarR/SlyA family transcriptional regulators, responsible for regulation of the biosynthesis of the sesquiterpenoid antibiotic pentalenolactone. The intrinsic target DNA sequences and small molecule ligands of purified recombinant PenR and PntR were identified by electrophoretic mobility shift assays. PenR bound to DNA from both the penR-gapN and penM-penH intergenic regions, while PntR bound only the corresponding pntR-gapR intergenic region. The targets of PenR and PntR were shown to be limited to conserved 37-bp DNA segments. Pentalenolactone and two late-stage biosynthetic intermediates, pentalenolactones D and F, act as ligands of both PenR and PntR, resulting in release of these proteins from their target DNA. The production of pentalenolactones was significantly decreased in the penR deletion mutant S. exfoliatus ΔpenR ZD27 but could be restored by complementation with either penR or pntR. Reverse transcription-PCR established that transcription of pentalenolactone biosynthetic and resistance genes decreased, while that of the penR gene itself increased in the penR deletion mutant S. exfoliatus ZD27 compared to the wild-type strain. The PenR protein thus serves as a positive regulator of pentalenolactone biosynthesis and self-resistance while acting as an autorepressor of penR.
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