ArticleCurrent protocols in human genetics2013
A multifaceted FISH approach to study endogenous RNAs and DNAs in native nuclear and cell structures.
Article in Current protocols in human genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Oligo(dT) Fluorescence In Situ Hybridization to Visualize the Poly(A) mRNAs in the Internal Tissues ofBio-protocol · 2026Article
- Impact of somatic XIST deletions on ongoing XIST expression and inactive X silencing and heterochromatin.Human molecular genetics · 2025Article
- Forced expression of MSR repeat transcripts above a threshold limit breaks heterochromatin organisation.Nature communications · 2025Article
- Locus-specific differential expression of human satellite sequences in the nuclei of cancer cells and heat-shocked cells.Nucleus (Austin, Tex.) · 2024Article
- Cytogenetic bands and sharp peaks of Alu underlie large-scale segmental regulation of nuclear genome architecture.Nucleus (Austin, Tex.) · 2024Article
- Differences in Alu vs L1-rich chromosome bands underpin architectural reorganization of the inactive-X chromosome and SAHFs.bioRxiv : the preprint server for biology · 2024Article
- Early chromosome condensation by XIST builds A-repeat RNA density that facilitates gene silencing.Cell reports · 2023Article
- Chromosome silencing in vitro reveals trisomy 21 causes cell-autonomous deficits in angiogenesis and early dysregulation in Notch signaling.Cell reports · 2022Article
- SAF-A mutants disrupt chromatin structure through dominant negative effects on RNAs associated with chromatin.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Article
- Reversal of RNA toxicity in myotonic dystrophy via a decoy RNA-binding protein with high affinity for expanded CUG repeats.Nature biomedical engineering · 2022Article
- Large-scale organoid study suggests effects of trisomy 21 on early fetal neurodevelopment are more subtle than variability between isogenic lines and experiments.Frontiers in neuroscience · 2022Article
- Nuclear envelope mechanobiology: linking the nuclear structure and function.Nucleus (Austin, Tex.) · 2021Review
- Ectopic expression of pericentric HSATII RNA results in nuclear RNA accumulation, MeCP2 recruitment, and cell division defects.Chromosoma · 2021Article
- Probing the function of long noncoding RNAs in the nucleus.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Review
- Silencing Trisomy 21 with XIST in Neural Stem Cells Promotes Neuronal Differentiation.Developmental cell · 2020Article
- lncRNA-Induced Spread of Polycomb Controlled by Genome Architecture, RNA Abundance, and CpG Island DNA.Molecular cell · 2019Article
- Trisomy silencing by XIST normalizes Down syndrome cell pathogenesis demonstrated for hematopoietic defects in vitro.Nature communications · 2018Article
- Trisomy 21 Represses Cilia Formation and Function.Developmental cell · 2018Article
- Demethylated HSATII DNA and HSATII RNA Foci Sequester PRC1 and MeCP2 into Cancer-Specific Nuclear Bodies.Cell reports · 2017Article
- Transient RUNX1 Expression during Early Mesendodermal Differentiation of hESCs Promotes Epithelial to Mesenchymal Transition through TGFB2 Signaling.Stem cell reports · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Fluorescence in situ hybridization (FISH) is not a singular technique, but a battery of powerful and versatile tools for examining the distribution of endogenous genes and RNAs in precise context with each other and in relation to specific proteins or cell structures. This unit offers the details of highly sensitive and successful protocols that were initially developed largely in our lab and honed over a number of years. Our emphasis is on analysis of nuclear RNAs and DNA to address specific biological questions about nuclear structure, pre-mRNA metabolism, or the role of noncoding RNAs; however, cytoplasmic RNA detection is also discussed. Multifaceted molecular cytological approaches bring precise resolution and sensitive multicolor detection to illuminate the organization and functional roles of endogenous genes and their RNAs within the native structure of fixed cells. Solutions to several common technical pitfalls are discussed, as are cautions regarding the judicious use of digital imaging and the rigors of analyzing and interpreting complex molecular cytological results.
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