ArticleApplied and environmental microbiology2013
Chemoreceptor VfcA mediates amino acid chemotaxis in Vibrio fischeri.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 39 citations in OpenAlex.
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- Lighting the way: how theJournal of bacteriology · 2024Review
- Vertical dynamics of free-living and particle-associatedFrontiers in microbiology · 2023Article
- Fine-Scale Structuring of PlanktonicApplied and environmental microbiology · 2022Article
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- A lasting symbiosis: how Vibrio fischeri finds a squid partner and persists within its natural host.Nature reviews. Microbiology · 2021Review
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- The Interaction of RecA With Both CheA and CheW Is Required for Chemotaxis.Frontiers in microbiology · 2020Article
- Ambient pH Alters the Protein Content of Outer Membrane Vesicles, Driving Host Development in a Beneficial Symbiosis.Journal of bacteriology · 2019Article
- The impact of Vibrio fischeri strain variation on host colonization.Current opinion in microbiology · 2019Review
- Article
- Methyl-accepting chemotaxis proteins: a core sensing element in prokaryotes and archaea.Cellular and molecular life sciences : CMLS · 2017Review
- A genomic comparison of 13 symbiotic Vibrio fischeri isolates from the perspective of their host source and colonization behavior.The ISME journal · 2016Article
- The Histidine Kinase BinK Is a Negative Regulator of Biofilm Formation and Squid Colonization.Journal of bacteriology · 2016Article
- Genome Evolution in the Obligate but Environmentally Active Luminous Symbionts of Flashlight Fish.Genome biology and evolution · 2016Article
- The periplasmic sensing domain of Vibrio fischeri chemoreceptor protein A (VfcA): cloning, purification and crystallographic analysis.Acta crystallographica. Section F, Structural biology communications · 2016Article
- Vibrio fischeri-derived outer membrane vesicles trigger host development.Cellular microbiology · 2016Article
- Characterization of the Vibrio fischeri Fatty Acid Chemoreceptors, VfcB and VfcB2.Applied and environmental microbiology · 2016Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Flagellar motility and chemotaxis by Vibrio fischeri are important behaviors mediating the colonization of its mutualistic host, the Hawaiian bobtail squid. However, none of the 43 putative methyl-accepting chemotaxis proteins (MCPs) encoded in the V. fischeri genome has been previously characterized. Using both an available transposon mutant collection and directed mutagenesis, we isolated mutants for 19 of these genes, and screened them for altered chemotaxis to six previously identified chemoattractants. Only one mutant was defective in responding to any of the tested compounds; the disrupted gene was thus named vfcA (Vibrio fischeri chemoreceptor A; locus tag VF_0777). In soft-agar plates, mutants disrupted in vfcA did not exhibit the serine-sensing chemotactic ring, and the pattern of migration in the mutant was not affected by the addition of exogenous serine. Using a capillary chemotaxis assay, we showed that, unlike wild-type V. fischeri, the vfcA mutant did not undergo chemotaxis toward serine and that expression of vfcA on a plasmid in the mutant was sufficient to restore the behavior. In addition to serine, we demonstrated that alanine, cysteine, and threonine are strong attractants for wild-type V. fischeri and that the attraction is also mediated by VfcA. This study thus provides the first insights into how V. fischeri integrates information from one of its 43 MCPs to respond to environmental stimuli.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.