ArticleApplied and environmental microbiology2013
Effects of DNA size on transformation and recombination efficiencies in Xylella fastidiosa.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 63 citations in OpenAlex.
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- Genomic divergence across the tree of life.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome.Nature biotechnology · 2023Article
- Shared properties of gene transfer agent and core genes revealed by comparative genomics of Alphaproteobacteria.Microbial genomics · 2022Article
- Development of the First Tractable Genetic System for Parvimonas micra, a Ubiquitous Pathobiont in Human Dysbiotic Disease.Microbiology spectrum · 2022Article
- Evolutionary Pathways and Trajectories in Antibiotic Resistance.Clinical microbiology reviews · 2021Article
- The role of interspecies recombination in the evolution of antibiotic-resistant pneumococci.eLife · 2021Article
- Orthology-Based Estimate of the Contribution of Horizontal Gene Transfer from Distantly Related Bacteria to the Intraspecific Diversity and Differentiation ofPathogens (Basel, Switzerland) · 2021Article
- Phenotypic Characterization and Transformation Attempts Reveal Peculiar Traits ofMicroorganisms · 2020Article
- CoreSimul: a forward-in-time simulator of genome evolution for prokaryotes modeling homologous recombination.BMC bioinformatics · 2020Article
- Structure and mechanism of DNA delivery of a gene transfer agent.Nature communications · 2020Article
- Secretion of a low and high molecular weight β-glycosidase by Yarrowia lipolytica.Microbial cell factories · 2020Article
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- Gene gain and loss push prokaryotes beyond the homologous recombination barrier and accelerate genome sequence divergence.Nature communications · 2019Article
- Complete genome sequence of Helicobacter pylori B128 7.13 and a single-step method for the generation of unmarked mutations.Helicobacter · 2019Article
- Plasmid Characteristics Modulate the Propensity of Gene Exchange in Bacterial Vesicles.Journal of bacteriology · 2019Article
- Mycoplasma Chromosomal Transfer: A Distributive, Conjugative Process Creating an Infinite Variety of Mosaic Genomes.Frontiers in microbiology · 2019Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Horizontally transferred DNA acquired through transformation and recombination has the potential to contribute to the diversity and evolution of naturally competent bacteria. However, many different factors affect the efficiency with which DNA can be transformed and recombined. In this study, we determined how the size of both homologous and nonhomologous regions affects transformation and recombination efficiencies in Xylella fastidiosa, a naturally competent generalist pathogen responsible for many emerging plant diseases. Our experimental data indicate that 96 bp of flanking homology is sufficient to initiate recombination, with recombination efficiencies increasing exponentially with the size of the homologous flanking region up to 1 kb. Recombination efficiencies also decreased with the size of the nonhomologous insert, with no recombination detected when 6 kb of nonhomologous DNA was flanked on either side by 1 kb of homologous sequences. Upon analyzing sequenced X. fastidiosa subsp. fastidiosa genomes for evidence of allele conversion, we estimated the mean size of recombination events to be 1,906 bp, with each event modifying, on average, 1.79% of the nucleotides in the recombined region. There is increasing evidence that horizontally acquired genes significantly affect the genetic diversity of X. fastidiosa, and DNA acquired through natural transformation could be a prominent mode of this horizontal transfer.
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