ArticleApplied and environmental microbiology2013
Isolation of a bifidogenic peptide from the pepsin hydrolysate of bovine lactoferrin.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- Oral Supplementation with Bovine Colostrum Decreases Intestinal Permeability and Stool Concentrations of Zonulin in Athletes.Nutrients · 2017Trial
- Growth and tolerance of formula with lactoferrin in infants through one year of age: double-blind, randomized, controlled trial.BMC pediatrics · 2015Trial
- Bioactive Peptides-Probiotics Interactions: Implications for Microbial Function and Human Health.Foods (Basel, Switzerland) · 2026Review
- Lactoferrin: Properties and Potential Uses in the Food Industry.International journal of molecular sciences · 2025Review
- Role of lactoferrin in osteopenia and osteoporosis.Frontiers in nutrition · 2025Review
- Lactoferrin and its digestive peptides induce interferon-α production and activate plasmacytoid dendritic cells ex vivo.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2023Article
- Clinical research review: usefulness of bovine lactoferrin in child health.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2023Review
- Oral administration of bovine lactoferrin suppresses the progression of rheumatoid arthritis in an SKG mouse model.PloS one · 2022Article
- Lactoferrin: A glycoprotein that plays an active role in human health.Frontiers in nutrition · 2022Review
- In the Age of Viral Pandemic, Can Ingredients Inspired by Human Milk and Infant Nutrition Be Repurposed to Support the Immune System?Nutrients · 2021Review
- Peptides from the Intestinal Tract of Breast Milk-Fed Infants Have Antimicrobial and Bifidogenic Activity.International journal of molecular sciences · 2021Article
- Milk Peptides Survive In Vivo Gastrointestinal Digestion and Are Excreted in the Stool of Infants.The Journal of nutrition · 2020Article
- Article
- The Impact of Lactoferrin on the Growth of Intestinal Inhabitant Bacteria.International journal of molecular sciences · 2019Review
- Milk Glycans and Their Interaction with the Infant-Gut Microbiota.Annual review of food science and technology · 2018Review
- The Impact of Gluten Friendly Flour on the Functionality of an Active Drink: Viability ofFrontiers in microbiology · 2018Article
- Impact of Gluten-Friendly Bread on the Metabolism and Function of In Vitro Gut Microbiota in Healthy Human and Coeliac Subjects.PloS one · 2016Article
- Regulatory effect of paraprobiotic Lactobacillus gasseri CP2305 on gut environment and function.Microbial ecology in health and disease · 2016Article
- Multifunctional iron bound lactoferrin and nanomedicinal approaches to enhance its bioactive functions.Molecules (Basel, Switzerland) · 2015Review
- The impact of the milk glycobiome on the neonate gut microbiota.Annual review of animal biosciences · 2015Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactoferrin is an iron-binding glycoprotein found in the milk of most mammals for which various biological functions have been reported, such as antimicrobial activity and bifidogenic activity. In this study, we compared the bifidogenic activity of bovine lactoferrin (bLF) and pepsin hydrolysate of bLF (bLFH), isolated bifidogenic peptide from bLFH, and investigated the bifidogenic spectra of bLF, bLFH, and its active peptide against 42 bifidobacterial strains comprising nine species. Against Bifidobacterium breve ATCC 15700(T), minimal effective concentrations of bLF and bLFH were 300 and 10 μg/ml. Against Bifidobacterium longum subsp. infantis ATCC 15697(T), the minimal effective concentration of bLFH was 30 μg/ml, and bLF did not show bifidogenic activity within 300 μg/ml. As an active peptide, a heterodimer of A(1)-W(16) and L(43)-A(48) linked by a disulfide bond was isolated. Previously, this peptide was identified as having antibacterial activity. An amino acid mixture with the same composition as this peptide showed no bifidogenic activity. The strains of each species whose growth was highly promoted (>150%) by this peptide at 3.75 μM were as follows: B. breve (7 out of 7 strains [7/7]), B. longum subsp. infantis (5/5), Bifidobacterium bifidum (2/5), B. longum subsp. longum (1/3), Bifidobacterium adolescentis (3/6), Bifidobacterium catenulatum (1/4), Bifidobacterium pseudocatenulatum (0/4), Bifidobacterium dentium (0/5), and Bifidobacterium angulatum (0/3). Growth of none of the strains was highly promoted by bLF at 3.75 μM. We demonstrated that bLFH showed stronger bifidogenic activity than natural bLF, especially against infant-representative species, B. breve and B. longum subsp. infantis; furthermore, we isolated its active peptide. This is the first report about a bifidogenic peptide derived from bLF.
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