ArticleEpigenetics2013
Hypoxia alters the epigenetic profile in cultured human placental trophoblasts.
Article in Epigenetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 3 of them syntheses that pooled it.
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Who cites it
44 citing papers in PubMed, 3 syntheses or guidelines pooled it, 84 citations in OpenAlex.
- A meta-analysis of pre-pregnancy maternal body mass index and placental DNA methylation identifies 27 CpG sites with implications for mother-child health.Communications biology · 2022Pooled it
- Current approaches and developments in transcript profiling of the human placenta.Human reproduction update · 2020Pooled it
- Gestational tissue transcriptomics in term and preterm human pregnancies: a systematic review and meta-analysis.BMC medical genomics · 2015Pooled it
- Hydrogen sulfide modulates gene networks in hypoxia/reoxygenation-stressed trophoblasts: insights from transcriptome profiling.Frontiers in bioinformatics · 2026Article
- Trophoblast adaptation to hypoxia: balance and dysfunction.Cell communication and signaling : CCS · 2025Review
- Molecular and Biochemical Mechanisms of Cardiomyopathy Development Following Prenatal Hypoxia-Focus on the NO System.Antioxidants (Basel, Switzerland) · 2025Review
- Combined analysis of the effects of hypoxia and oxidative stress on DNA methylation and the transcriptome in HTR-8/SVneo trophoblast cells.Journal of cellular and molecular medicine · 2024Article
- Maternal haemoglobin levels in pregnancy and child DNA methylation: a study in the pregnancy and childhood epigenetics consortium.Epigenetics · 2022Article
- Epigenetic analysis in placentas from sickle cell disease patients reveals a hypermethylation profile.PloS one · 2022Article
- The Impact of Hypoxia in Early Pregnancy on Placental Cells.International journal of molecular sciences · 2021Review
- Article
- Cell-specific characterization of the placental methylome.BMC genomics · 2021Article
- The role of N-cadherin/c-Jun/NDRG1 axis in the progression of prostate cancer.International journal of biological sciences · 2021Article
- Urothelial Cancer Associated 1 (UCA1) and miR-193 Are Two Non-coding RNAs Involved in Trophoblast Fusion and Placental Diseases.Frontiers in cell and developmental biology · 2021Article
- ZNF217: the cerberus who fails to guard the gateway to lethal malignancy.American journal of cancer research · 2021Review
- Omics Approaches to Study Formation and Function of Human Placental Syncytiotrophoblast.Frontiers in cell and developmental biology · 2021Review
- The significance of the placental genome and methylome in fetal and maternal health.Human genetics · 2020Review
- H3K4me3-Mediated Upregulation of LncRNA-HEIPP in Preeclampsia Placenta Affects Invasion of Trophoblast Cells.Frontiers in genetics · 2020Article
- Transcriptome-wide analysis of the SCNT bovine abnormal placenta during mid- to late gestation.Scientific reports · 2019Article
- Identification of diagnostic biomarkers in patients with gestational diabetes mellitus based on transcriptome gene expression and methylation correlation analysis.Reproductive biology and endocrinology : RB&E · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The mechanisms by which the placenta adapts to exogenous stimuli to create a stable and healthy environment for the growing fetus are not well known. Low oxygen tension influences placental function, and is associated with preeclampsia, a condition displaying altered development of placental trophoblast. We hypothesized that oxygen tension affects villous trophoblast by modulation of gene expression through DNA methylation. We used the Infinium HumanMethylation450 BeadChip array to compare the DNA methylation profile of primary cultures of human cytotrophoblasts and syncytiotrophoblasts under < 1%, 8% and 20% oxygen levels. We found no effect of oxygen tension on average DNA methylation for either cell phenotype, but a set of loci became hypermethylated in cytotrophoblasts exposed for 24 h to < 1% oxygen, as compared with those exposed to 8% or 20% oxygen. Hypermethylation with low oxygen tension was independently confirmed by bisulfite-pyrosequencing in a subset of functionally relevant genes including CD59, CFB, GRAM3 and ZNF217. Intriguingly, 70 out of the 147 CpGs that became hypermethylated in < 1% oxygen overlapped with CpG sites that became hypomethylated upon differentiation of cytotrophoblasts into syncytiotrophoblasts. Furthermore, the preponderance of altered sites was located at AP-1 binding sites. We suggest that AP-1 expression is triggered by hypoxia and interacts with DNA methyltransferases (DNMTs) to target methylation at specific sites in the genome, thus causing suppression of the associated genes that are responsible for differentiation of villous cytotrophoblast to syncytiotrophoblast.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.