ArticleAnesthesiology2013
Dual effects of isoflurane on proliferation, differentiation, and survival in human neuroprogenitor cells.
Article in Anesthesiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
56 citing papers in PubMed, 105 citations in OpenAlex.
- Calcium Signaling Dysregulation as a Convergent Mechanism in Anesthetic-Induced Developmental Neurotoxicity.Drug design, development and therapy · 2026Review
- High-Dose Propofol Induces Cytotoxicity by Elevating Intracellular CaDrug design, development and therapy · 2026Article
- The antioxidative effect of STAT3 involved in cellular vulnerability to isoflurane.BMC neuroscience · 2024Article
- The Effects of Sevoflurane and Aβ Interaction on CA1 Dendritic Spine Dynamics and MEGF10-Related Astrocytic Synapse Engulfment.International journal of molecular sciences · 2024Article
- HSP90 Inhibition Attenuated Isoflurane-Induced Neurotoxicity in Mice and Human Neuroglioma Cells.Neurochemical research · 2024Article
- Suberoylanilide hydroxamic acid attenuates cognitive impairment in offspring caused by maternal surgery during mid-pregnancy.PloS one · 2024Article
- Arrestin domain-containing protein 1-mediated microvesicles (ARMMs) protect against cadmium-induced neurotoxicity.Extracellular vesicle · 2023Article
- Pluripotent stem cell-derived neural progenitor cells can be used to model effects of IL-6 on human neurodevelopment.Disease models & mechanisms · 2023Article
- Sevoflurane Induces a Cyclophilin D-Dependent Decrease of Neural Progenitor Cells Migration.International journal of molecular sciences · 2023Article
- Sevoflurane Exposure of Clinical Doses in Pregnant Rats Induces Vcan Changes without Significant Neural Apoptosis in the Offspring.Medicina (Kaunas, Lithuania) · 2023Article
- MicroRNA-424-5p Alleviates Isoflurane Anesthesia-Induced Neurotoxicity in Human Embryonic Stem Cell-Derived Neurons by Targeting FASN.Computational and mathematical methods in medicine · 2022Article
- Role of GABAFrontiers in cellular neuroscience · 2022Article
- Article
- An appropriate level of autophagy reduces emulsified isoflurane-induced apoptosis in fetal neural stem cells.Neural regeneration research · 2020Article
- Induced pluripotent stem cells as a platform to understand patient-specific responses to opioids and anaesthetics.British journal of pharmacology · 2020Review
- Sevoflurane modulates breast cancer cell survival via modulation of intracellular calcium homeostasis.BMC anesthesiology · 2020Article
- Maternal sevoflurane exposure affects differentiation of hippocampal neural stem cells by regulating miR-410-3p and ATN1.Stem cell research & therapy · 2020Article
- A Guide to Understanding "State-of-the-Art" Basic Research Techniques in Anesthesiology.Anesthesia and analgesia · 2020Review
- GABAInternational journal of molecular sciences · 2020Article
- Conflicting Actions of Inhalational Anesthetics, Neurotoxicity and Neuroprotection, Mediated by the Unfolded Protein Response.International journal of molecular sciences · 2020Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundPrevious studies have demonstrated that isoflurane can provide both neuroprotection and neurotoxicity in various tissue culture models and in rodent developing brains. The cellular and molecular mechanisms mediating these dual effects are not clear, but the exposure level and duration of isoflurane appear to be determinant factors.
methodsUsing the ReNcell CX (Millipore, Billerica, MA) human neural progenitor cell line, the authors investigated the impact of prolonged exposure to varying isoflurane concentrations on cell survival and neurogenesis. In addition, the authors assessed the impact of short isoflurane preconditioning on elevation of cytosolic Ca concentration and cytotoxic effects mediated by prolonged isoflurane exposures and the contribution of inositol-1,4,5-trisphosphate or ryanodine receptor activation to these processes.
resultsShort exposures to low isoflurane concentrations promote proliferation and differentiation of ReNcell CX cells, with no cell damage. However, prolonged exposures to high isoflurane concentrations induced significant ReNcell CX cell damage and inhibited cell proliferation. These prolonged exposures suppressed neuronal cell fate and promoted glial cell fate. Preconditioning of ReNcell CX cultures with short exposures to low concentrations of isoflurane ameliorated the effects of prolonged exposures to isoflurane. Pretreatment of ReNcell cultures with inositol-1,4,5-trisphosphate or ryanodine receptor antagonists mostly prevented isoflurane-mediated effects on survival, proliferation, and differentiation. Finally, isoflurane-preconditioned cultures showed significantly less isoflurane-evoked changes in calcium concentration.
conclusionThe commonly used general anesthetic isoflurane exerts dual effects on neuronal stem cell survival, proliferation, and differentiation, which may be attributed to differential regulation of calcium release through activation of endoplasmic reticulum localized inositol-1,4,5-trisphosphate and/or ryanodine receptors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.