ArticleBone2013
Early detection of burn induced heterotopic ossification using transcutaneous Raman spectroscopy.
Article in Bone, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 1 of them a synthesis that pooled it.
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Who cites it
54 citing papers in PubMed, 1 synthesis or guideline pooled it, 86 citations in OpenAlex.
- The intelligent lift: Artificial Intelligence's growing role in plastic surgery - a comprehensive review.Frontiers in surgery · 2025Pooled it
- VEGF-D-induced intraosseous lymphangiogenesis drives site-specific heterotopic bone resorption.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Early detection of aberrant cell fate and repair using circulating progenitor cells in patients with heterotopic ossification.Nature communications · 2026Article
- Carnosic acid serves as a dual Nrf2 activator and PTEN/AKT suppressor to inhibit traumatic heterotopic ossification.Stem cell research & therapy · 2025Article
- Elucidating the Mechanism of Radiation Therapy on Mesenchymal Cell Fate in Preventing Heterotopic Ossification.Annals of surgery · 2025Article
- The HIF-1α/PLOD2 axis integrates extracellular matrix organization and cell metabolism leading to aberrant musculoskeletal repair.Bone research · 2024Article
- MicroRNA-138: an emerging regulator of skeletal development, homeostasis, and disease.American journal of physiology. Cell physiology · 2023Review
- Discoidin domain receptor 2 regulates aberrant mesenchymal lineage cell fate and matrix organization.Science advances · 2022Article
- Macrophage TGF-β signaling is critical for wound healing with heterotopic ossification after trauma.JCI insight · 2022Article
- Acetabular Reaming Is a Reliable Model to Produce and Characterize Periarticular Heterotopic Ossification of the Hip.Stem cells translational medicine · 2022Article
- Perspective on the integration of optical sensing into orthopedic surgical devices.Journal of biomedical optics · 2022Article
- Measures of Bone Mineral Carbonate Content and Mineral Maturity/Crystallinity for FT-IR and Raman Spectroscopic Imaging Differentially Relate to Physical-Chemical Properties of Carbonate-Substituted Hydroxyapatite.Calcified tissue international · 2021Article
- Calcitonin Gene-Related Peptide Influences Bone-Tendon Interface Healing Through Osteogenesis: Investigation in a Rabbit Partial Patellectomy Model.Orthopaedic journal of sports medicine · 2021Article
- High Frequency Spectral Ultrasound Imaging Detects Early Heterotopic Ossification in Rodents.Stem cells and development · 2021Article
- Metformin attenuates trauma-induced heterotopic ossification via inhibition of Bone Morphogenetic Protein signalling.Journal of cellular and molecular medicine · 2020Article
- Immobilization after injury alters extracellular matrix and stem cell fate.The Journal of clinical investigation · 2020Article
- Endogenous CCN family member WISP1 inhibits trauma-induced heterotopic ossification.JCI insight · 2020Article
- Investigation into Possible Association of Oxandrolone and Heterotopic Ossification Following Burn Injury.Journal of burn care & research : official publication of the American Burn Association · 2019Article
- External Bone Size Is a Key Determinant of Strength-Decline Trajectories of Aging Male Radii.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2019Article
- Heterotopic Ossification: A Comprehensive Review.JBMR plus · 2019Review
Corrections and comments
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
Abstract
introductionHeterotopic ossification (HO), or the abnormal formation of bone in soft tissue, occurs in over 60% of major burn injuries and blast traumas. A significant need exists to improve the current diagnostic modalities for HO which are inadequate to diagnose and intervene on HO at early time-points. Raman spectroscopy has been used in previous studies to report on changes in bone composition during bone development but has not yet been applied to burn induced HO. In this study, we validate transcutaneous, in-vivo Raman spectroscopy as a methodology for early diagnosis of HO in mice following a burn injury.
methodsAn Achilles tenotomy model was used to study HO formation. Following tenotomy, mice were divided into burn and sham groups with exposure of 30% surface area on the dorsum to 60° water or 30° water for 18s respectively. In-vivo, transcutaneous Raman spectroscopy was performed at early time points (5 days, 2 and 3 weeks) and a late time point (3 months) on both the tenotomized and non-injured leg. These same samples were then dissected down to the bone and ex-vivo Raman measurements were performed on the excised tissue. Bone formation was verified with Micro CT and histology at corresponding time-points.
resultsOur Raman probe allowed non-invasive, transcutaneous evaluation of heterotopic bone formation. Raman data showed significantly increased bone mineral signaling in the tenotomy compared to control leg at 5 days post injury, with the difference increasing over time whereas Micro CT did not demonstrate heterotopic bone until three weeks. Ex-vivo Raman measurements showed significant differences in the amount of HO in the burn compared to sham groups and also showed differences in the spectra of new, ectopic bone compared to pre-existing cortical bone.
conclusionsBurn injury increases the likelihood of developing HO when combined with traumatic injury. In our in-vivo mouse model, Raman spectroscopy allowed for detection of HO formation as early as 5 days post injury. Changes in bone mineral and matrix composition of the new bone were also evidenced in the Raman spectra which could facilitate early identification of HO and allow more timely therapy decisions for HO patients.
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Registered trials
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