ArticleJournal of virological methods2013
Development of a multiplex one step RT-PCR that detects eighteen respiratory viruses in clinical specimens and comparison with real time RT-PCR.
Article in Journal of virological methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.
- Nucleic acid amplification tests on respiratory samples for the diagnosis of coronavirus infections: a systematic review and meta-analysis.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2021Pooled it
- Human Metapneumovirus (HMPV): Advances in Diagnosis, Molecular Epidemiology, and Clinical Impact of an Underrecognized Respiratory Virus.Diagnostics (Basel, Switzerland) · 2026Review
- Reclassification of respiratory syncytial virus genotypes in India.Virusdisease · 2023Article
- Respiratory syncytial virus among children hospitalized with severe acute respiratory infection in Kashmir, a temperate region in northern India.Journal of global health · 2022Observational
- Application of MRT-qPCR for pathogen detection of lower respiratory tract infection.American journal of translational research · 2022Article
- Evaluation of one-step RT-PCR multiplex assay for body fluid identification.International journal of legal medicine · 2021Article
- The Burden of Respiratory Viruses and Their Prevalence in Different Geographical Regions of India: 1970-2020.Frontiers in microbiology · 2021Review
- Disease Burden Due to Respiratory Syncytial Virus in Indian Pediatric Population: A Literature Review.Clinical medicine insights. Pediatrics · 2021Review
- Duplex real-time RT-PCR assay for detection and subgroup-specific identification of human respiratory syncytial virus.Journal of virological methods · 2019Article
- Detection of six common human paramyxoviruses in patients with acute febrile respiratory symptoms using a novel multiplex real-time RT-PCR assay.Journal of medical virology · 2019Article
- Simultaneous detection of 15 respiratory pathogens with a fluorescence probe melting curve analysis-based multiplex real-time PCR assay.International journal of molecular epidemiology and genetics · 2019Article
- The Application Analysis of Multiplex Real-Time Polymerase Chain Reaction Assays for Detection of Pathogenic Bacterium in Peritoneal Dialysis-Associated Peritonitis.Blood purification · 2019Article
- A novel multiplex PCR for virus detection by melting curve analysis.Journal of virological methods · 2018Article
- A Multiplex Asymmetric Reverse Transcription-PCR Assay Combined With an Electrochemical DNA Sensor for Simultaneously Detecting and Subtyping Influenza A Viruses.Frontiers in microbiology · 2018Article
- A Rare Case of Human Coronavirus 229E Associated with Acute Respiratory Distress Syndrome in a Healthy Adult.Case reports in infectious diseases · 2018Article
- Viral Pneumonia in Patients with Hematopoietic Cell Transplantation and Hematologic Malignancies.Clinics in chest medicine · 2017Review
- Recent sequence variation in probe binding site affected detection of respiratory syncytial virus group B by real-time RT-PCR.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2017Article
- A Melting Curve-Based Multiplex RT-qPCR Assay for Simultaneous Detection of Four Human Coronaviruses.International journal of molecular sciences · 2016Article
- Prevalence and Seasonal Distribution of Respiratory Viruses During the 2014 - 2015 Season in Istanbul.Jundishapur journal of microbiology · 2016Article
- Metapneumovirus Infections and Respiratory Complications.Seminars in respiratory and critical care medicine · 2016Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid and accurate diagnosis of viral respiratory infections is crucial for patient management. Multiplex reverse transcriptase polymerase chain reaction (mRT-PCR) is used increasingly to diagnose respiratory infections and has shown to be more sensitive than viral culture and antigen detection. Objective of the present study was to develop a one-step mRT-PCR that could detect 18 respiratory viruses in three sets. The method was compared with real time RT-PCR (rRT-PCR) for its sensitivity and specificity. Clinical specimens from 843 pediatric patients with respiratory symptoms were used in the study. 503 (59.7%) samples were detected positive by mRT-PCR. Of these 462 (54.8%) exhibited presence of a single pathogen and 41 (4.9%) had multiple pathogens. rRT-PCR detected 439 (52.1%) positive samples, where 419 (49.7%) exhibited one virus and 20 (2.4%) showed co-infections. Concordance between mRT-PCR and rRT-PCR was 91.9% and kappa correlation 0.837. Sensitivity and specificity of mRT-PCR were 99.5% and 83.7% while that of rRT-PCR was 86.9% and 99.4% respectively. Rhinovirus (17.2%) was the most frequently detected virus followed by respiratory syncytial virus B (15.4%), H1N1pdm09 (8.54%), parainfluenza virus-3 (5.8%) and metapneumovirus (5.2%). In conclusion, mRT-PCR is a rapid, cost effective, specific and highly sensitive method for detection of respiratory viruses.
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