ArticleJournal of neuroscience methods2013
Advances in thin tissue Golgi-Cox impregnation: fast, reliable methods for multi-assay analyses in rodent and non-human primate brain.
Article in Journal of neuroscience methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Dendritic pathology and overexpression of MAP2 in Purkinje cells from mice inoculated with rabies virus.Journal of molecular histology · 2024Article
- Unpredictable chronic mild stress shows neuronal remodeling in multipolar projection neurons of hippocampal complex in postnatal chicks.Anatomical science international · 2024Article
- Neuronal plasticity in hippocampal neurons due to chronic mild stress and after stress removal in postnatal chicks.Journal of anatomy · 2024Article
- Article
- Quantification of Neuronal Dendritic Spine Density and Lengths of Apical and Basal Dendrites in Temporal Lobe Structures Using Golgi-Cox Staining.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Quinpirole inhibits levodopa-induced dyskinesias at structural and behavioral levels: Efficacy negated by co-administration of isradipine.Experimental neurology · 2023Article
- Immunotherapy with Cleavage-Specific 12A12mAb Reduces the Tau Cleavage in Visual Cortex and Improves Visuo-Spatial Recognition Memory in Tg2576 AD Mouse Model.Pharmaceutics · 2023Article
- Acute stress-induced neuronal plasticity in the corticoid complex of 15-day-old chick, Gallus domesticus.Journal of anatomy · 2021Article
- Preclinical evidence in support of repurposing sub-anesthetic ketamine as a treatment for L-DOPA-induced dyskinesia.Experimental neurology · 2020Article
- Striatal Nurr1, but not FosB expression links a levodopa-induced dyskinesia phenotype to genotype in Fisher 344 vs. Lewis hemiparkinsonian rats.Experimental neurology · 2020Article
- Striatal Nurr1 Facilitates the Dyskinetic State and Exacerbates Levodopa-Induced Dyskinesia in a Rat Model of Parkinson's Disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Optimized Golgi-Cox Staining Validated in the Hippocampus of Spared Nerve Injury Mouse Model.Frontiers in neuroanatomy · 2020Article
- Genetic silencing of striatal CaV1.3 prevents and ameliorates levodopa dyskinesia.Movement disorders : official journal of the Movement Disorder Society · 2019Article
- Overexpression of neuronal KThe journal of physiological sciences : JPS · 2019Article
- Modernization of Golgi staining techniques for high-resolution, 3-dimensional imaging of individual neurons.Scientific reports · 2019Article
- Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method.Journal of visualized experiments : JoVE · 2017Article
- Colony-stimulating factor 1 receptor blockade prevents fractionated whole-brain irradiation-induced memory deficits.Journal of neuroinflammation · 2016Article
- Optimising Golgi-Cox staining for use with perfusion-fixed brain tissue validated in the zQ175 mouse model of Huntington's disease.Journal of neuroscience methods · 2016Article
- Golgi-Cox Staining Step by Step.Frontiers in neuroanatomy · 2016Article
- Interrogating the aged striatum: robust survival of grafted dopamine neurons in aging rats produces inferior behavioral recovery and evidence of impaired integration.Neurobiology of disease · 2015Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
In 1873 Camillo Golgi discovered a staining technique that allowed for the visualization of whole neurons within the brain, initially termed 'the black reaction' and is now known as Golgi impregnation. Despite the capricious nature of this method, Golgi impregnation remains a widely used method for whole neuron visualization and analysis of dendritic arborization and spine quantification. We describe a series of reliable, modified 'Golgi-Cox' impregnation methods that complement some existing methods and have several advantages over traditional whole brain 'Golgi' impregnation. First, these methods utilize 60-100μm thick brain sections, which allows for fast, reliable impregnation of neurons in rats (7-14 days) and non-human primates (NHP) (30 days) while avoiding the pitfalls of other 'rapid Golgi' techniques traditionally employed with thin sections. Second, these methods employ several common tissue fixatives, resulting in high quality neuron impregnation in brain sections from acrolein, glutaraldehyde, and paraformaldehyde perfused rats, and in glutaraldehyde perfused NHP brain tissue. Third, because thin sections are obtained on a vibratome prior to processing, alternate sections of brain tissue can be used for additional analyses such as immunohistochemistry or electron microscopy. This later advantage allows for comparison of, for example, dendrite morphology in sections adjacent to pertinent histochemical markers or ultrastructural components. Finally, we describe a method for simultaneous light microscopic visualization of both tyrosine hydroxylase immunohistochemistry and Golgi impregnation in the same tissue section. Thus, the methods described here allow for fast, high quality Golgi impregnation and conserve experimental subjects by allowing multiple analyses within an individual animal.
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