ArticleCellular microbiology2013
Cryptosporidium parvum scavenges LDL-derived cholesterol and micellar cholesterol internalized into enterocytes.
Article in Cellular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- Harnessing cholesterol uptake of malaria parasites for therapeutic applications.EMBO molecular medicine · 2024Article
- Modulation of Cholesterol Pathways in Human Macrophages Infected by Clinical Isolates ofFrontiers in cellular and infection microbiology · 2022Article
- Novel Insights Into Sterol Uptake and Intracellular Cholesterol Trafficking DuringFrontiers in cellular and infection microbiology · 2022Article
- Thiosemicarbazone Copper Chelator BLT-1 Blocks Apicomplexan Parasite Replication by Selective Inhibition of Scavenger Receptor B Type 1 (SR-BI).Microorganisms · 2021Article
- Ezetimibe blocksParasitology · 2021Article
- P-Glycoprotein Inhibitors Differently AffectPathogens (Basel, Switzerland) · 2021Article
- Malaria Parasites Hijack Host Receptors From Exosomes to Capture Lipoproteins.Frontiers in cell and developmental biology · 2021Article
- Article
- ATP6V0d2 controls Leishmania parasitophorous vacuole biogenesis via cholesterol homeostasis.PLoS pathogens · 2019Article
- Besnoitia besnoiti infection alters both endogenous cholesterol de novo synthesis and exogenous LDL uptake in host endothelial cells.Scientific reports · 2019Article
- Discovery of Novel Anti-cryptosporidial Activities From Natural Products byFrontiers in microbiology · 2019Article
- Changes to cholesterol trafficking in macrophages by Leishmania parasites infection.MicrobiologyOpen · 2017Article
- Metabolic signatures of Besnoitia besnoiti-infected endothelial host cells and blockage of key metabolic pathways indicate high glycolytic and glutaminolytic needs of the parasite.Parasitology research · 2016Article
- Cardiovascular disease and type 2 diabetes in evolutionary perspective: a critical role for helminths?Evolution, medicine, and public health · 2016Review
- Host Lipid Bodies as Platforms for Intracellular Survival of Protozoan Parasites.Frontiers in immunology · 2016Review
- Eimeria bovis infection modulates endothelial host cell cholesterol metabolism for successful replication.Veterinary research · 2015Article
- Neospora caninum Recruits Host Cell Structures to Its Parasitophorous Vacuole and Salvages Lipids from Organelles.Eukaryotic cell · 2015Article
- A nanotherapy strategy significantly enhances anticryptosporidial activity of an inhibitor of bifunctional thymidylate synthase-dihydrofolate reductase from Cryptosporidium.Bioorganic & medicinal chemistry letters · 2015Article
- Isoprenoid metabolism in apicomplexan parasites.Current clinical microbiology reports · 2014Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Cryptosporidium spp. are responsible for devastating diarrhoea in immunodeficient individuals. In the intestinal tract, the developmental stages of the parasite are confined to the apical surfaces of epithelial cells. Upon invasion, Cryptosporidium incorporates the microvillous membrane of the enterocyte to form the parasitophorous vacuole (PV) and sequesters itself from the host cytoplasm by rearranging the host cytoskeleton. Cryptosporidium parvum has minimal anabolic capabilities and relies on transporters and salvage pathways to meet its basic metabolic requirements. The cholesterol salvage pathway is crucial for the development of protozoan parasites. In this study, we have examined the sources of cholesterol from C. parvum infecting enterocytes. We illustrated that the intracellular stages of Cryptosporidium as well as the oocysts shed by the host, contain cholesterol. Incubation of infected enterocytes in lipoprotein-free medium impairs parasite development and results in substantial decrease in cholesterol content associated with the PV. Among lipoproteins, LDL constitutes an important source of cholesterol for Cryptosporidium. Dietary cholesterol incorporated into micelles is internalized into enterocytes by the NPC1L1 transporter. We showed that C. parvum also obtains cholesterol from micelles in enterocytes.Pharmacological blockade of NPC1L1 function by ezetimibe or moderate downregulation of NPC1L1 expression decreases parasite infectivity. These observations indicate that, despite its dual sequestration from the intestinal lumen and the host cytoplasm, C. parvum can, in fact, obtain cholesterol both from the gut's lumen and the host cell. This study highlights the evolutionary advantages for epicellular pathogens to access to nutrients from the outside and inside of the host cell.
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