ArticleBMC cell biology2013
mTOR direct interactions with Rheb-GTPase and raptor: sub-cellular localization using fluorescence lifetime imaging.
Article in BMC cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 66 citations in OpenAlex.
- Targeting kinases that regulate programmed cell death: a new therapeutic strategy for breast cancer.Journal of translational medicine · 2025Review
- Spatial and functional separation of mTORC1 signalling in response to different amino acid sources.Nature cell biology · 2024Article
- The SapM phosphatase can arrest phagosome maturation in an ESX-1 independent manner inInfection and immunity · 2024Article
- ATP homeostasis and signaling in plants.Plant communications · 2024Review
- Low temperature and mTOR inhibition favor stem cell maintenance in human keratinocyte cultures.EMBO reports · 2023Article
- Hierarchical inhibition of mTORC1 by glucose starvation-triggered AXIN lysosomal translocation and by AMPK.Life metabolism · 2023Article
- Arf5-mediated regulation of mTORC1 at the plasma membrane.Molecular biology of the cell · 2023Article
- Compartmentalization, a key mechanism controlling the multitasking role of the SnRK1 complex.Journal of experimental botany · 2022Review
- Article
- An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.Molecular cell · 2021Article
- VHL suppresses RAPTOR and inhibits mTORC1 signaling in clear cell renal cell carcinoma.Scientific reports · 2021Article
- Regulation of mTORC1 activity by the Golgi apparatus.Faculty reviews · 2021Review
- The Multifaceted Role of Nutrient Sensing and mTORC1 Signaling in Physiology and Aging.Frontiers in aging · 2021Review
- Multicolor FRET-FLIM Microscopy to Analyze Multiprotein Interactions in Live Cells.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Directly imaging the localisation and photosensitization properties of the pan-mTOR inhibitor, AZD2014, in living cancer cells.Journal of photochemistry and photobiology. B, Biology · 2020Article
- Review
- Automated Fluorescence Lifetime Imaging High-Content Analysis of Förster Resonance Energy Transfer between Endogenously Labeled Kinetochore Proteins in Live Budding Yeast Cells.SLAS technology · 2019Article
- Ataxia-Telangiectasia Mutated is located in cardiac mitochondria and impacts oxidative phosphorylation.Scientific reports · 2019Article
- Direct imaging of the recruitment and phosphorylation of S6K1 in the mTORC1 pathway in living cells.Scientific reports · 2019Article
- Cryo-EM insight into the structure of MTOR complex 1 and its interactions with Rheb and substrates.F1000Research · 2019Review
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
Abstract
backgroundThe mammalian target of rapamycin (mTOR) signalling pathway has a key role in cellular regulation and several diseases. While it is thought that Rheb GTPase regulates mTOR, acting immediately upstream, while raptor is immediately downstream of mTOR, direct interactions have yet to be verified in living cells, furthermore the localisation of Rheb has been reported to have only a cytoplasmic cellular localization.
resultsIn this study a cytoplasmic as well as a significant sub-cellular nuclear mTOR localization was shown , utilizing green and red fluorescent protein (GFP and DsRed) fusion and highly sensitive single photon counting fluorescence lifetime imaging microscopy (FLIM) of live cells. The interaction of the mTORC1 components Rheb, mTOR and raptor, tagged with EGFP/DsRed was determined using fluorescence energy transfer-FLIM. The excited-state lifetime of EGFP-mTOR of ~2400 ps was reduced by energy transfer to ~2200 ps in the cytoplasm and to 2000 ps in the nucleus when co-expressed with DsRed-Rheb, similar results being obtained for co-expressed EGFP-mTOR and DsRed-raptor. The localization and distribution of mTOR was modified by amino acid withdrawal and re-addition but not by rapamycin.
conclusionsThe results illustrate the power of GFP-technology combined with FRET-FLIM imaging in the study of the interaction of signalling components in living cells, here providing evidence for a direct physical interaction between mTOR and Rheb and between mTOR and raptor in living cells for the first time.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.