ArticleAntioxidants & redox signaling2013
Effects of erythrocyte aging on nitric oxide and nitrite metabolism.
Article in Antioxidants & redox signaling, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Influence of Atherosclerotic Risk Factors on the Effectiveness of Therapeutic Ultrasound Cavitation for Flow Augmentation.Journal of the American Society of Echocardiography : official publication of the American Society of Echocardiography · 2024Article
- Peroxiredoxin-2 recycling is slower in denser and pediatric sickle cell red cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Storage-Induced Micro-Erythrocytes Can Be Quantified and Sorted by Flow Cytometry.Frontiers in physiology · 2022Article
- Aging-Induced Impairment of Vascular Function: Mitochondrial Redox Contributions and Physiological/Clinical Implications.Antioxidants & redox signaling · 2021Review
- Human and rodent red blood cells do not demonstrate xanthine oxidase activity or XO-catalyzed nitrite reduction to NO.Free radical biology & medicine · 2021Article
- An Overview of NO Signaling Pathways in Aging.Molecules (Basel, Switzerland) · 2021Review
- Red cell distribution width is associated with all-cause mortality in patients with acute stroke: a retrospective analysis of a large clinical database.The Journal of international medical research · 2021Article
- Red blood cell storage lesion: causes and potential clinical consequences.Blood transfusion = Trasfusione del sangue · 2019Review
- Effect of medicinal mushrooms on blood cells under conditions of diabetes mellitus.World journal of diabetes · 2017Review
- Association between alcohol-induced erythrocyte membrane alterations and hemolysis in chronic alcoholics.Journal of clinical biochemistry and nutrition · 2017Article
- Learning more and spending less with neglected laboratory parameters: the paradigmatic case of red blood cell distribution width.Acta bio-medica : Atenei Parmensis · 2016Review
- Red blood cell washing, nitrite therapy, and antiheme therapies prevent stored red blood cell toxicity after trauma-hemorrhage.Free radical biology & medicine · 2015Article
- Article
- Heritability of glutathione and related metabolites in stored red blood cells.Free radical biology & medicine · 2014Article
- Mechanism of faster NO scavenging by older stored red blood cells.Redox biology · 2014Article
- Oridonin Attenuates the Effects of Chronic Alcohol Consumption Inducing Oxidative, Glycative and Inflammatory Injury in the Mouse Liver.In vivo (Athens, Greece)Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
aimsRecent studies have suggested that in addition to oxygen transport, red blood cells (RBC) are key regulators of vascular function by both inhibiting and promoting nitric oxide (NO)-mediated vasodilation. Most studies assume that RBC are homogenous, but, in fact, they comprise cells of differing morphology and biochemical composition which are dependent on their age, parameters that control NO reactions. We tested the hypothesis that distinct RBC populations will have differential effects on NO signaling.
resultsYoung and old RBC were separated by density gradient centrifugation. Consistent with previous reports, old RBC had decreased levels of surface N-acetyl neuraminic acid and increased oxygen binding affinities. Competition kinetic experiments showed that older RBCs scavenged NO∼2-fold faster compared with younger RBC, which translated to a more potent inhibition of both acetylcholine and NO-donor dependent vasodilation of isolated aortic rings. Moreover, nitrite oxidation kinetics was faster with older RBC compared with younger RBC; whereas no differences in nitrite-reduction kinetics were observed. This translated to increased inhibitory effect of older RBC to nitrite-dependent vasodilation under oxygenated and deoxygenated conditions. Finally, leukodepleted RBC storage also resulted in more dense RBC, which may contribute to the greater NO-inhibitory potential of stored RBC. INNOVATION: These results suggest that a key element in vascular NO-homeostasis mechanisms is the distribution of RBC ages across the physiological spectrum (0-120 days) and suggest a novel mechanism for inhibited NO bioavailability in diseases which are characterized by a shift to an older RBC phenotype.
conclusionOlder RBC inhibit NO bioavailability by increasing NO- and nitrite scavenging.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.