ArticleCilia2012
A method for measuring electrical signals in a primary cilium.
Article in Cilia, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Molecular pathology and cystogenic propensity of the ADPKD Taiwan founder variant.JCI insight · 2025Article
- Pathogenic variants in the polycystin pore helix cause distinct forms of channel dysfunction.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Hyperosmolality activates polycystin-2 and TRPM4 in renal primary cilium.Pflugers Archiv : European journal of physiology · 2025Article
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- A synthetic method to assay polycystin channel biophysics.bioRxiv : the preprint server for biology · 2024Article
- Role of the microtubules in the electrical activity of the primary cilium of renal epithelial cells.Frontiers in molecular biosciences · 2023Article
- Regenerative Calcium Currents in Renal Primary Cilia.Frontiers in physiology · 2022Article
- Inward CaAmerican journal of physiology. Renal physiology · 2021Article
- Disrupting polycystin-2 EF hand CaJournal of cell science · 2020Article
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- Article
- Article
- Primary cilia regulate the osmotic stress response of renal epithelial cells through TRPM3.American journal of physiology. Renal physiology · 2017Article
- The native TRPP2-dependent channel of murine renal primary cilia.American journal of physiology. Renal physiology · 2017Article
- Progress in ciliary ion channel physiology.The Journal of general physiology · 2017Review
- Calcium channels in primary cilia.Current opinion in nephrology and hypertension · 2016Review
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- A TRPM4-dependent current in murine renal primary cilia.American journal of physiology. Renal physiology · 2015Article
- Electrical Signaling in Motile and Primary Cilia.Bioscience · 2014Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMost cells in the body possess a single primary cilium. These cilia are key transducers of sensory stimuli, and defects in cilia have been linked to several diseases. Evidence suggests that some transduction of sensory stimuli by the primary cilium depends on ion-conducting channels. However, the tiny size of the cilium has been a critical barrier to understanding its electrical properties. We report a novel method that allows sensitive, repeatable electrical recordings from primary cilia. Adherent cells were grown on small, spherical beads that could be easily moved within the recording chamber. In this configuration, an entire cilium could be pulled into a recording microelectrode.
resultsIn 47% of attempts, suction resulted in a seal with high input resistance. Single channels could be recorded while the cilium remained attached to the cell. When the pipette was raised into the air, the cell body was pulled off at the air-bath interface. The pipette retained the cilium and could then be immersed in various solutions that bathed the cytoplasmic face of the membrane. In excised cilia, ionic currents through ciliary channels were modulated by cytoplasmic Ca(2+) and transmembrane voltage.
conclusionsCiliary recording is a direct way to learn the effects of second messengers and voltage changes on ciliary transduction channels.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.