ArticlePloS one2013
The NifA-RpoN regulon of Mesorhizobium loti strain R7A and its symbiotic activation by a novel LacI/GalR-family regulator.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- Unveiling the Functions of Two RpoNs inInternational journal of molecular sciences · 2026Article
- CPOP1 is a key enzyme required for nodule microenvironment control and successful symbiotic nitrogen fixation in Lotus japonicus.Journal of integrative plant biology · 2025Article
- Exploring the cellular surface polysaccharide and root nodule symbiosis characteristics of theMicrobiology spectrum · 2023Article
- Article
- Role of two RpoN inFrontiers in microbiology · 2023Article
- Production of the plant hormone gibberellin by rhizobia increases host legume nodule size.The ISME journal · 2022Article
- Revealing potential functions of hypothetical proteins induced by genistein in the symbiosis island of Bradyrhizobium japonicum commercial strain SEMIA 5079 (= CPAC 15).BMC microbiology · 2022Article
- Comparative analysis of integrative and conjugative mobile genetic elements in the genusMicrobial genomics · 2021Article
- How Rhizobia Adapt to the Nodule Environment.Journal of bacteriology · 2021Article
- Symbiosis islands of Loteae-nodulatingMicrobial genomics · 2020Article
- OptimizingProceedings of the National Academy of Sciences of the United States of America · 2020Article
- NifA is the master regulator of both nitrogenase systems in Rhodobacter capsulatus.MicrobiologyOpen · 2019Article
- Positive and negative regulation of transferred nif genes mediated by indigenous GlnR in Gram-positive Paenibacillus polymyxa.PLoS genetics · 2018Article
- Rhizobia: from saprophytes to endosymbionts.Nature reviews. Microbiology · 2018Review
- Regulation of Nitrogen Fixation inFrontiers in microbiology · 2018Article
- Occurrence of myo-inositol and alkyl-substituted polysaccharide in the prey-trapping mucilage of Drosera capensis.Die Naturwissenschaften · 2017Article
- The hidden life of integrative and conjugative elements.FEMS microbiology reviews · 2017Review
- Characterization of CYP115 As a Gibberellin 3-Oxidase Indicates That Certain Rhizobia Can Produce Bioactive Gibberellin AACS chemical biology · 2017Article
- LacI Transcriptional Regulatory Networks in Clostridium thermocellum DSM1313.Applied and environmental microbiology · 2017Article
- Nitrogen Fixation and Molecular Oxygen: Comparative Genomic Reconstruction of Transcription Regulation in Alphaproteobacteria.Frontiers in microbiology · 2016Article
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Mesorhizobium loti is the microsymbiont of Lotus species, including the model legume L. japonicus. M. loti differs from other rhizobia in that it contains two copies of the key nitrogen fixation regulatory gene nifA, nifA1 and nifA2, both of which are located on the symbiosis island ICEMlSym(R7A). M. loti R7A also contains two rpoN genes, rpoN1 located on the chromosome outside of ICEMlSym(R7A) and rpoN2 that is located on ICEMlSym(R7A). The aims of the current work were to establish how nifA expression was activated in M. loti and to characterise the NifA-RpoN regulon. The nifA2 and rpoN2 genes were essential for nitrogen fixation whereas nifA1 and rpoN1 were dispensable. Expression of nifA2 was activated, possibly in response to an inositol derivative, by a novel regulator of the LacI/GalR family encoded by the fixV gene located upstream of nifA2. Other than the well-characterized nif/fix genes, most NifA2-regulated genes were not required for nitrogen fixation although they were strongly expressed in nodules. The NifA-regulated nifZ and fixU genes, along with nifQ which was not NifA-regulated, were required in M. loti for a fully effective symbiosis although they are not present in some other rhizobia. The NifA-regulated gene msi158 that encodes a porin was also required for a fully effective symbiosis. Several metabolic genes that lacked NifA-regulated promoters were strongly expressed in nodules in a NifA2-dependent manner but again mutants did not have an overt symbiotic phenotype. In summary, many genes encoded on ICEMlSym(R7A) were strongly expressed in nodules but not free-living rhizobia, but were not essential for symbiotic nitrogen fixation. It seems likely that some of these genes have functional homologues elsewhere in the genome and that bacteroid metabolism may be sufficiently plastic to adapt to loss of certain enzymatic functions.
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